DOHH-2 BioVector®难治性人免疫母细胞性B细胞淋巴瘤细胞系human B-cell lymphoma line

价　　格：¥99860
货　　号：BioVector®DOHH-2
产　　地：北京

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BioVector® DOHH-2（常写作 DoHH2）是一种人 B 细胞淋巴瘤细胞系。该细胞系于 1990 年建立，取自一名 60 岁男性患者的胸腔积液，该患者患有从滤泡性中心细胞/中心母细胞淋巴瘤演变为难治性免疫母细胞性 B 细胞淋巴瘤。

核心特征

疾病来源：难治性免疫母细胞性 B 细胞淋巴瘤（转化自滤泡性淋巴瘤）。
遗传学特征：具有典型的 t(14;18)(q32;q21) 染色体易位，导致 IGH-BCL2 融合基因形成，表现为 BCL2 蛋白高表达。
EB 病毒 (EBV) 状态： EBV 阴性。
形态：圆形，呈悬浮生长。

培养与维护指南

参数	规格要求
培养基	RPMI 1640 + 10% 胎牛血清 (FBS)
培养条件	37°C，5% CO₂
倍增时间	约 40–48 小时
接种密度	建议起始密度 0.5 x 10⁶ cells/mL；维持在 0.3–0.7 x 10⁶ cells/mL
最高密度	0.7–1.0 x 10⁶ cells/mL
传代比例	1:2，每隔 2 天传代一次
冻存液	70% 培养基 + 20% FBS + 10% DMSO

免疫表型

DOHH-2 细胞表达以下标志物：CD10, CD19, CD20, CD37, CD38, HLA-DR。不表达 CD3, CD13, CD34 和 CD138。

主要研究应用

凋亡研究：由于 BCL2 高表达，常用于评估 BCL2 抑制剂（如 Venetoclax）的药效。
耐药性研究：常被用于构建伊布替尼（Ibrutinib）耐药模型。
异种移植物 (Xenograft)：可用于免疫缺陷小鼠的体内成瘤实验。

BioVector®

The DOHH-2 (also written as DoHH2) cell line is a human B-cell lymphoma line established in 1990 from the pleural effusion of a 60-year-old male patient with refractory immunoblastic B-cell lymphoma. It is widely used in oncology research, particularly for studying follicular lymphoma (FL) and diffuse large B-cell lymphoma (DLBCL).

Core Characteristics

Disease Origin: The cell line originated from refractory immunoblastic B-cell lymphoma that progressed from follicular centroblastic/centrocytic lymphoma.
Genetic Features: DOHH-2 cells exhibit the characteristic t(14;18)(q32;q21) translocation, leading to the IGH-BCL2 fusion gene and overexpression of BCL2.
EBV Status: It is an EBV-negative cell line.
Morphology: Cells are generally round and grow in suspension.

Culture & Maintenance

Parameter	Specification
Medium	RPMI 1640 supplemented with 10% heat-inactivated FBS
Incubation	37°C with 5% CO₂
Doubling Time	Approximately 40–48 hours
Seeding Density	Seed at ~0.5 x 10⁶ cells/mL; maintain at 0.3–0.7 x 10⁶ cells/mL
Max Density	0.7–1.0 x 10⁶ cells/mL
Split Ratio	1:2 every second day
Storage	Frozen in 70% medium, 20% FBS, 10% DMSO

Immunophenotype

DOHH-2 cells express markers such as CD10, CD19, CD20, CD37, CD38, and HLA-DR. They are negative for markers including CD3, CD13, CD34, and CD138.

Key Research Applications

Apoptosis Studies: Useful for evaluating BCL2 inhibitors due to high BCL2 expression.
Drug Resistance: Employed to develop ibrutinib-resistant models.
Xenografts: Suitable for in vivo studies in immunocompromised mice.

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

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