首页 » SMG-C6 BioVector® 大鼠下颌腺腺泡永生化细胞系Rat Submandibular Gland-Clone 6 cell line-用于牙科，药理学研究

SMG-C6 BioVector® 大鼠下颌腺腺泡永生化细胞系Rat Submandibular Gland-Clone 6 cell line-用于牙科，药理学研究

  • 价　　格：¥99850
  • 货　　号：BioVector®SMG-C6
  • 产　　地：北京
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BioVector® SMG-C6 (Submandibular Gland-Clone 6) cell line is a well-established immortalized rat salivary gland acinar cell line used primarily in dental and physiological research.

Core Specifications

  • Origin: Rat (Rattus norvegicus), submandibular salivary gland.

  • Cell Type: Salivary gland acinar cell (epithelial-like).

  • Transformation: Immortalized by Simian virus 40 (SV40).

  • Morphology: Epithelial; typically grows as an adherent monolayer.

Culture Conditions
To maintain SMG-C6 cells in a laboratory setting, the following parameters are standard:

  • Basal Medium: Often a 1:1 mixture of DMEM/Ham's F-12 medium.

  • Supplements:

    • 5–10% Fetal Bovine Serum (FBS).

    • Glutamine (2 mM).

    • Additional growth factors (e.g., insulin, EGF, hydrocortisone, and transferrin) are sometimes added to maintain specialized acinar functions.


  • Environment: 37°C in a humidified atmosphere containing 5% CO₂.

  • Subculturing: Passaged using standard 0.25% Trypsin-EDTA when cells reach 80–90% confluency.

Key Biological Features

  • Tight Junctions: SMG-C6 cells are known for their ability to form functional tight junctions, characterized by high Transepithelial Electrical Resistance (TER) values and the expression of proteins like ZO-1 and occludin.

  • Acinar Function: They retain properties of polarized acinar cells, making them a preferred model for studying salivary secretion, ion transport, and paracellular permeability.

  • Applications: Widely used to research sialadenitis (salivary gland inflammation), the effects of pharmaceutical agents on saliva production, and therapeutic delivery systems like nanoparticles.

Safety & Handling

  • Biosafety Level: BSL-2 (due to SV40 transformation).

  • Storage: Long-term storage in liquid nitrogen vapor phase using a cryopreservation medium (e.g., 90% FBS + 10% DMSO).

BioVector®SMG-C6（大鼠下颌腺克隆6）是一种广泛应用于牙科、生理学及药理学研究的永生化细胞系。

核心参数

  • 来源： 大鼠（Rattus norvegicus）下颌唾液腺。

  • 细胞类型： 唾液腺腺泡细胞（类上皮细胞）。

  • 永生化方式： 通过 猿猴病毒40 (SV40) 转染实现。

  • 形态： 上皮细胞状；贴壁生长。

培养条件

  • 基础培养基： 通常使用 DMEM/Ham's F-12 (1:1) 混合培养基。

  • 添加剂：

    • 5–10% 胎牛血清 (FBS)。

    • 2 mM 谷氨酰胺。

    • 有时需添加胰岛素、表皮生长因子 (EGF)、氢化可的松和转铁蛋白，以维持其腺泡分泌功能。


  • 生长环境： 37°C，5% CO₂ 湿润培养箱。

  • 传代： 细胞汇合度达到 80–90% 时，使用 0.25% Trypsin-EDTA 进行消化传代。

Salidroside mitigated radiation damage in SMG cells. SMG-C6 cells were used in (A) and (B). SMG-C6 cells were pretreated with salidroside (5, 10, or 20 μM) for 24 h before exposure to 15 Gy X-ray and tested at 24, 48, and 72 h after irradiation, respectively. (A) Representative images of SMG-C6 cells were obtained by an inverted microscope (bar = 100 μm). (B) Cell viability was detected with a CCK-8 assay. The data are presented as the mean ± SEM from 3 independent experiments, with each sample analyzed in triplicate. ** p < 0.01 vs. the control; # p < 0.05 and ## p < 0.01 vs. irradiation alone.

SMG-C6 cells were stimulated with 1, 5, and 10 μg/ml fAd (A) or 0.25, 0.5, 1, and 2 μg/ml gAd (B) for 10 min, respectively. The levels of p-AMPK were detected by Western blot analysis. GAPDH expression was used as an internal control. Values are means ± SD from 4 independent experiments. *P<0.05 and **P<0.01 compared with control. Time course of TER was measured by an epithelial volt ohm meter. (C) SMG-C6 cells were incubated with 5 μg/ml fAd or 1 μg/ml gAd for the indicated times. (D) SMG-C6 cells were incubated with 1 mM AICAR or treated with 1 mM AraA for 30 min and followed by fAd, gAd, or AraA alone. Values are means ± SD from 4 independent experiments. *P<0.05 and **P<0.01 compared with untreated control cells.

主要生物学特征

  • 紧密连接： SMG-C6 能够形成功能性紧密连接，具有较高的 跨上皮电阻 (TER) 值，并表达 ZO-1 和 Occludin 等蛋白。

  • 极性特征： 该细胞保留了极化腺泡细胞的特性，是研究唾液分泌、离子转运和旁细胞渗透性的理想模型。

  • 应用领域： 广泛用于研究唾液腺炎症（如干燥综合征相关研究）、药物对唾液分泌的影响，以及口腔药物递送系统的开发。

安全与处理

  • 生物安全等级： BSL-2（因含有 SV40 基因序列）。

  • 冻存： 建议储存于液氮中；冻存液通常为 90% 胎牛血清 + 10% DMSO。

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