hNav1.8/β1 BioVector® cell line人钠离子通道 α 亚基 (Nav1.8) 和 β1 亚基重组稳定细胞系-用于离子通道活性分析和药物筛选

价　　格：¥998950
货　　号：BioVector® hNav1.8/β1
产　　地：北京

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BioVector® hNav1.8/β1 重组稳定细胞系主要用于离子通道活性分析和药物筛选，特别是针对人类钠离子通道 α 亚基 (Nav1.8) 和 β1 亚基的研究。以下是基于BioVector NTCC典型培养物保藏中心的数据表摘要：

细胞系基本规格

宿主细胞： HEK-293 (或其高贴壁变体 293H)。
靶点蛋白：人源 Nav1.8 (alpha 亚基) 和人源 β1 (辅助亚基)。
应用领域：全细胞膜片钳 (Patch-clamp) 电生理实验、荧光法离子通道分析、以及自动化膜片钳 (APC) 系统。
供应形式：通常为 2 支冷冻细胞管。

电生理特性

激活特性：该通道在大约 -40 mV 时开始激活，并在 20 mV 左右达到峰值电流。
半激活电压 (V½)：测量值约为 -1.2 ± 0.7 mV。
斜率因子 (k)：记录为 11.4 ± 0.6。
动力学特征：产生具有缓慢失活 (slow inactivation) 特性的内向电流，这是 Nav1.8 通道的典型特征。

实验表现与优势

β1 亚基的作用：辅助亚基 β1 的共同表达显著增加了峰值电流幅度，并增强了钠通道在细胞表面的表达。
稳定性：该细胞系在多代培养中保持稳定的表达水平，确保了长期药物筛选实验的重复性。
药理学验证：经过验证，该细胞系对已知的钠通道阻断剂（如四毒素 TTX，Nav1.8 通常表现为 TTX 耐受型）具有预期的反应。

Characterization of Endogenous Sodium Channels in the ND7-23 Neuroblastoma Cell Line:Implications for Use as a Heterologous Ion Channel Expression System Suitable for Automated Patch Clamp Screening | ASSAY and Drug Development

A novel selective and orally bioavailable <fc>N</fc>a<fc><sub>v</sub></fc>1.8 channel blocke

The BioVector® hNav1.8/β1 Recombinant Stable Cell Line is designed for ion channel activity assays and drug discovery, specifically targeting the human sodium channel alpha subunit (Nav1.8) and the beta 1 subunit (β1). The datasheet for the widely utilized BioVector NTCC Inc. includes the following specifications:

Cell Line Specifications

Host Cell Line: 293H (a variant of HEK-293 designed for better adherence in assays).
Target Channels: Human Nav1.8 (alpha subunit) and Human β1 (beta subunit).
Application: Whole-cell patch-clamp electrophysiology, ion channel activity assays, and automated patch-clamp (APC) systems.
Quantity: Typically supplied as 2 vials of frozen cells.

Electrophysiological Properties

Activation Threshold: The channel activates at approximately -40 mV, reaching peak current at 20 mV.
Half-Activation Voltage (V½): Measured at -1.2 ± 0.7 mV.
Slope Factor (k): Recorded as 11.4 ± 0.6.
Kinetics: Activation produces an inward current with slow inactivation kinetics, which is characteristic of the Nav1.8 channel.

Assay Performance

Role of β1 Subunit: The inclusion of the β1 subunit is critical as it increases peak current amplitude and enhances the expression of the sodium ion channel at the cell surface.
Stability: These lines provide stable expression over many generations, allowing for reproducible data in long-term studies.

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

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