ST9671 BioVector®高产植物抗氧化剂白藜芦醇的解脂耶罗维亚酵母菌株resveratrol

NTCC® ST9671 是一种经基因改造的解脂耶罗维亚酵母（Yarrowia lipolytica）菌株，专门用于高产植物抗氧化剂——白藜芦醇（resveratrol）。

• 宿主物种：解脂耶罗维亚酵母 (Yarrowia lipolytica)，这是一种非模式产油酵母，在生物工程中被广泛用作“细胞工厂”。

• 保存编号：该菌株已保存在 Euroscarf 菌种库，访问编号为 Y41418。

• 衍生关系：它是由亲本菌株 ST6512 改造而来的。

• 多拷贝集成：整合了 5 个拷贝的白藜芦醇生物合成基因，包括来自植物和细菌的 FjTAL、At4CL1 和 VvVST1。

• 解除反馈抑制：过表达了莽草酸途径中的关键基因突变体（YlARO4K221L 和 YlARO7G139S），以消除天然代谢调节对产量的限制。

• 白藜芦醇产量：在小规模培养中产量可达 409-525 mg/L。

• 发酵罐表现：在受控的补料分罐发酵（fed-batch）中，其产量高达 12.4 g/L，这是目前报道的该类化合物从头合成（de novo production）的最高水平之一。

BioVector® ST9671 strain is a genetically engineered yeast (Yarrowia lipolytica) developed for the high-level production of the plant antioxidant resveratrol and other aromatic compounds. The strain has been deposited with the Euroscarf collection under accession number Y41418.

• Host Organism: The strain is a non-conventional yeast, Yarrowia lipolytica, derived from the parental strain ST6512.

• Genetic Modifications: It was engineered by integrating five additional copies of the resveratrol biosynthetic genes (FjTAL, At4CL1, and VvVST1) and overexpressing feedback-insensitive alleles of the key genes in the shikimate pathway (YlARO4K221L and YlARO7G139S).

• Function: ST9671 is a top-producing strain engineered to produce high levels of resveratrol, reaching a titer of up to 409.0 mg/L in small-scale cultivation and 12.4 g/L in a controlled fed-batch bioreactor, which was the highest reported titer for de novo resveratrol production at the time of the study.

• Research Context: This strain is primarily used in metabolic engineering research to explore the potential of Yarrowia lipolytica as a host organism for producing commercially viable levels of valuable metabolites for industries like food, medical, and cosmetic applications.