- BioVector NTCC典型培养物保藏中心
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[Designation名称] NTCC® Synonyms: U251-GFP Cell Line细胞株
BioVector NTCC Inc.Synonyms细胞别名: Cell Line Name: U251-hrGFPComments: Population: Caucasian.NTCC® Accession Number: CVCL_E9DUReferences identifiers: PubMed=32591581;Comments: Characteristics: Using CRISPR/Cas9 a CAG-hrGFP-PuroR construct was introduced in the AAVS1 safe harbor locus (PubMed=32591581).Comments: Sequence variation: Mutation; HGNC; HGNC:9588; PTEN; Simple; p.Glu242Valfs*15 (c.723_724dupTG); ClinVar=VCV000536553; Zygosity=Unspecified (from parent cell line).Comments: Sequence variation: Mutation; HGNC; HGNC:11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).Comments: Sequence variation: Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg273His (c.818G>A); ClinVar=VCV000012366; Zygosity=Unspecified (from parent cell line).Comments: Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; 84CGO; rrGFP (Note=hrGFP; luorescent protein published in 2007, green fluorescent protein derived from R.reniformis).Comments: Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).Comments: Derived from site: In situ; Brain, parietal lobe; UBERON=UBERON_0001872.Diseases: C60781; AstrocytomaDiseases: Orphanet_94; AstrocytomaSpecies of origin: NCBI_TaxID=9606; ! Homo sapiens (Human)Hierarchy: CVCL_0021 ! U-251MG
[Organism物种来源]
[Disease疾病种类]
[Accession编号] NTCC®-NTCC® Accession Number: CVCL_E9DU
[Comments描述]
[Reference参考文献]
[Category分类]
[Date保藏时间]
[Hierarchy]
培养方法：
Medium培养基：NTCC® Synonyms: U251-GFP Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.Atmosphere: 95% Air, 5% CO2
Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
Website网址：www.biovector.net
BioVector NTCC Inc.Synonyms细胞别名: Cell Line Name: U251-hrGFPComments: Population: Caucasian.NTCC® Accession Number: CVCL_E9DUReferences identifiers: PubMed=32591581;Comments: Characteristics: Using CRISPR/Cas9 a CAG-hrGFP-PuroR construct was introduced in the AAVS1 safe harbor locus (PubMed=32591581).Comments: Sequence variation: Mutation; HGNC; HGNC:9588; PTEN; Simple; p.Glu242Valfs*15 (c.723_724dupTG); ClinVar=VCV000536553; Zygosity=Unspecified (from parent cell line).Comments: Sequence variation: Mutation; HGNC; HGNC:11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).Comments: Sequence variation: Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg273His (c.818G>A); ClinVar=VCV000012366; Zygosity=Unspecified (from parent cell line).Comments: Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; 84CGO; rrGFP (Note=hrGFP; luorescent protein published in 2007, green fluorescent protein derived from R.reniformis).Comments: Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).Comments: Derived from site: In situ; Brain, parietal lobe; UBERON=UBERON_0001872.Diseases: C60781; AstrocytomaDiseases: Orphanet_94; AstrocytomaSpecies of origin: NCBI_TaxID=9606; ! Homo sapiens (Human)Hierarchy: CVCL_0021 ! U-251MG
[Organism物种来源]
[Disease疾病种类]
[Accession编号] NTCC®-NTCC® Accession Number: CVCL_E9DU
[Comments描述]
[Reference参考文献]
[Category分类]
[Date保藏时间]
[Hierarchy]
培养方法：
Medium培养基：NTCC® Synonyms: U251-GFP Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.Atmosphere: 95% Air, 5% CO2
Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
Website网址：www.biovector.net
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