KT2440恶臭假单胞菌NTCC® (Pseudomonas putida) BioVector NTCC保藏中心

BioVector® NTCC® KT2440恶臭假单胞菌NTCC® (Pseudomonas putida)

The KT2440 strain is a prominent, non-pathogenic strain of the soil bacterium Pseudomonas putida. It is widely recognized in industrial biotechnology and environmental science as a robust, safe chassis for genetic engineering, metabolic studies, and bioremediation applications. Description and Characteristics Organism: Pseudomonas putida. Strain Designation: KT2440 (also known by collection numbers DSM 6125 or ATCC 47054). Origin: It is a derivative of the parental toluene-degrading bacterium P. putida mt-2, which was originally isolated from soil in 1963. The KT2440 strain was created by removing the large pWW0 TOL plasmid. Primary Use: Functions as a robust model organism and host for recombinant DNA experiments, industrial bioproduction, and environmental bioremediation projects. Morphology: It is a Gram-negative, rod-shaped bacterium. Metabolism: Highly versatile; it possesses the metabolic pathways to utilize a wide range of carbon sources, including many complex aromatic compounds. Oxygen Requirement: It is an obligate aerobe, requiring oxygen for growth. Optimal Temperature: It is a mesophile that grows best at 30°C. Biosafety Level: It has been certified by the NIH as a safe Host-Vector Biosafety Level 1 (HV1) system, suitable for use in standard laboratory P1 or ML1 environments. Research Applications Non-Pathogenic: Its lack of virulence factors (such as the type III secretion system found in related pathogens like P. aeruginosa) was a key reason it was certified as a safe laboratory strain for genetic engineering. Bioremediation: Its ability to tolerate and metabolize a variety of toxic chemicals and complex carbon sources makes it ideal for converting industrial waste into valuable products. Synthetic Biology: A large number of genetic tools (plasmids, transposons) have been developed for its manipulation, making it an effective "chassis" organism for synthetic biology applications. Culture Conditions Culture Medium: Standard Luria–Bertani (LB) broth or agar plates are typically used for general laboratory growth. Minimal media (e.g., M9 minimal salts medium) can be used when researchers need to supplement specific carbon sources like citrate or glucose. Temperature: Cultures should be grown at 30°C for optimal growth. Aeration: Requires adequate aeration and aerobic conditions for growth. KT2440 菌株是恶臭假单胞菌 (Pseudomonas putida) 中一种著名、无致病性的土壤细菌菌株。它在工业生物技术和环境科学中被广泛认可，是一种健壮、安全的基因工程、代谢研究和生物修复应用的底盘生物。 描述和特征 生物体： Pseudomonas putida (恶臭假单胞菌)。 菌株名称： KT2440（也称为收藏号 DSM 6125 或 ATCC 47054）。 来源： 它是耐甲苯降解的亲代恶臭假单胞菌 mt-2 菌株的衍生物，mt-2 于 1963 年从土壤中分离得到。KT2440 菌株是通过去除 pWW0 甲苯分解 (TOL) 质粒而产生的。 主要用途： 用作模型生物宿主，用于重组 DNA 实验、工业生物生产和环境生物修复项目。 形态： 它是革兰氏阴性、杆状细菌。 新陈代谢： 具有高度的多功能性；它拥有利用多种碳源（包括许多复杂的芳香族化合物）的代谢途径。 氧气需求： 它是专性需氧菌，生长需要氧气。 最适温度： 它是中温生物，在 30°C 时生长最佳。 生物安全级别： 已被 NIH 认证为安全的宿主-载体生物安全 1 级 (HV1) 系统，适合在标准 P1 或 ML1 实验室环境中使用。 研究应用 无致病性： 它缺乏毒力因子（如在相关病原体铜绿假单胞菌中发现的 III 型分泌系统），这是其被认证为安全的重组 DNA 工作实验室菌株的关键原因。 代谢多功能性： 它能够耐受和代谢多种有毒化学物质和复杂碳源，非常适合将工业废物转化为有价值的产品（生物修复/生物催化）。 遗传易操作性： 已经开发了大量的遗传工具（质粒、转座子）用于其操作，使其成为合成生物学应用的有效“底盘”生物。 培养条件 培养基： 标准的 Luria–Bertani (LB) 肉汤或琼脂平板通常用于普通实验室培养。当研究人员需要补充特定的碳源（如柠檬酸盐或葡萄糖）时，可以使用最小培养基（例如 M9 最小盐培养基）。 温度： 培养物应在 30°C 下生长以获得最佳生长速度。 通气： 需要足够的通气和有氧条件才能生长。 BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心 电话:400-800-2947 工作QQ/微信同号:1843439339 网址 www.biovector.net

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