BWP17 致病性酵母菌白色念珠菌BioVector® (Candida albicans) BioVector NTCC保藏中心

BioVector® NTCC® BWP17 致病性酵母菌白色念珠菌BioVector® (Candida albicans)

The BWP17 strain is a widely used laboratory strain of the pathogenic yeast Candida albicans. It is a derivative of the well-characterized clinical isolate SC5314 and is an essential tool for molecular genetic studies due to specific auxotrophic markers that allow for highly efficient gene manipulation and selection processes using methods like Ura-blaster. Description and Characteristics Organism: Candida albicans. Strain Designation: BWP17; it is also cataloged as MYA-2543 by the ATCC. Origin: Derived through genetic manipulation of the parental SC5314 clinical isolate. Primary Use: A workhorse strain for gene deletion (knockout) and gene complementation studies in C. albicans research. Morphology: This organism is a dimorphic fungus, meaning it can grow in two forms: as budding yeast (blastospores) or as filamentous hyphae. Biosafety Level: Typically handled under BSL-1 or BSL-2 safety guidelines, as C. albicans is a common human pathogen. Genetic Features (Auxotrophies) The BWP17 strain is defined by homozygous deletions in three key genes, meaning it cannot produce these nutrients itself and requires them in the culture medium: ura3::λimm434/ura3::λimm434: Requires supplementation with uracil (or uridine). his1::hisG/his1::hisG: Requires supplementation with histidine. arg4::hisG/arg4::hisG: Requires supplementation with arginine. Culture and Handling Culture Medium: General Growth: YPAD (Yeast Peptone Dextrose medium supplemented with adenine) is standard rich media. Selection: Synthetic Complete (SC) media lacking specific amino acids (e.g., SC -His, SC -Arg) are used to select for organisms that have taken up a complementing plasmid. Growth Temperature: Optimal growth in the yeast form occurs at 30°C. Hyphal Induction: To study the pathogenic filamentous (hyphal) form of growth, cells are typically shifted to 37°C in specific inducing media (e.g., YPD + 5% Fetal Bovine Serum). BWP17 菌株是致病性酵母菌白色念珠菌 (Candida albicans) 的一种广泛使用的实验室菌株。它是经过充分表征的临床分离株 SC5314 的衍生物，由于具有多个营养缺陷型标记，可以通过 Ura-blaster 等方法实现高效的基因操作和筛选过程，因此是分子遗传学研究的重要工具。 描述和特征 生物体： Candida albicans (白色念珠菌)。 菌株名称： BWP17；在 ATCC 中也编目为 MYA-2543。 来源： 通过对亲代 SC5314 临床分离株进行基因操作而衍生。 主要用途： 是白色念珠菌研究中进行基因缺失（敲除）和基因互补研究的主力菌株。 形态： 这种生物体是一种双态性真菌，意味着它可以以两种形式生长：出芽酵母菌（芽生孢子）或丝状菌丝体。 生物安全级别： 由于白色念珠菌是常见的人类病原体，通常在 BSL-1 或 BSL-2 安全指南下进行处理。 遗传特征（营养缺陷型） BWP17 菌株的定义是三个关键生物合成基因存在纯合缺失，这意味着它无法自行产生这些营养物质，需要在培养基中额外补充： ura3::λimm434/ura3::λimm434： 需要补充尿嘧啶（或尿苷）。 his1::hisG/his1::hisG： 需要补充组氨酸。 arg4::hisG/arg4::hisG： 需要补充精氨酸。 培养和处理 培养基： 通用培养基： YPAD（补充腺嘌呤的酵母蛋白胨葡萄糖培养基）是标准的富集培养基。 最小/选择性培养基： 缺乏特定氨基酸的合成完全培养基 (SC)（例如 SC -His、SC -Arg）用于筛选携带互补质粒的转化体。 生长温度： 酵母菌形式的最佳生长温度为 30°C。 菌丝诱导： 为了研究致病性丝状（菌丝）生长形式，通常将细胞转移到 37°C 的特定诱导培养基（例如 YPD + 5% 胎牛血清）中孵育。 BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心 电话:400-800-2947 工作QQ/微信同号:1843439339 网址 www.biovector.net

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