pRT802大肠杆菌链霉菌整合型和穿梭载体比他 BioVector NTCC保藏中心

BioVector® NTCC® pRT802大肠杆菌链霉菌整合型和穿梭载体比他

The pRT802 plasmid is a specialized integrative expression vector primarily designed for use in Streptomyces species of bacteria, which are important for producing antibiotics. It allows researchers to insert genes into the bacterial genome at a specific, stable location. Key Features Vector Type: Integrative vector and shuttle vector (E. coli - Streptomyces). Host Organisms: Used for cloning in Escherichia coli and for functional use in a wide range of Streptomyces strains. Bacterial Resistance: Confers kanamycin resistance (typically used at 50 µg/mL). Integration System: Contains the site-specific integration machinery (integrase gene and attP attachment site) from the temperate phage φBT1. Origins of Replication: ColE1 origin (pBR322oriF): Allows the plasmid to replicate and be maintained in E. coli. It cannot replicate autonomously in Streptomyces. Instead, it integrates into the Streptomyces genome at the φBT1 attB site. Other Features: Includes a multiple cloning site (MCS) for inserting a gene of interest and an origin of conjugative transfer (oriT), which facilitates its transfer via tri-parental mating into Streptomyces. Usage in Research The pRT802 vector is commonly used in Streptomyces research for several applications: Gene Expression: To drive the expression of cloned genes within the Streptomyces host. Complementation: To reintroduce a functional copy of a gene into a mutant strain. Strain Marking: To produce stably marked strains for experimental purposes. Gene Deletion/Targeting: It is used as a backbone for constructing plasmids that facilitate gene knockout experiments via homologous recombination. pRT802 质粒是一种特殊的整合表达载体，主要设计用于链霉菌属 (Streptomyces) 细菌，该菌属因产生抗生素而非常重要。它允许研究人员将基因插入细菌基因组的特定稳定位置。 主要特征 载体类型： 整合型载体和穿梭载体（大肠杆菌 - 链霉菌）。 宿主生物体： 用于在大肠杆菌中进行克隆，并用于多种链霉菌菌株中发挥功能。 细菌抗性： 赋予卡那霉素耐药性（通常使用 50 µg/mL）。 整合系统： 包含来自温和噬菌体 φBT1 的位点特异性整合机制（整合酶基因和 attP 附着位点）。 复制起点： ColE1 起点 (pBR322oriF)： 允许质粒在大肠杆菌中复制和维持。 它不能在链霉菌中自主复制。相反，它通过 φBT1 attB 位点整合到链霉菌基因组中。 其他特征： 包括一个多克隆位点 (MCS) 用于插入目标基因，以及一个接合转移起点 (oriT)，便于通过三亲交配将其转移到链霉菌中。 研究用途 pRT802 载体通常用于链霉菌研究中的多种应用： 基因表达： 驱动克隆基因在链霉菌宿主中的表达。 互补： 将功能性基因拷贝重新引入突变菌株中。 菌株标记： 用于生产稳定的标记菌株以用于实验目的。 基因删除/靶向： 它被用作构建质粒的骨架，通过同源重组促进基因敲除实验。 BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心 电话:400-800-2947 工作QQ/微信同号:1843439339 网址 www.biovector.net

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