NK5449细菌接合实验受体大肠杆菌菌株BioVector® BioVector NTCC保藏中心

BioVector® NTCC® NK5449细菌接合实验受体大肠杆菌菌株BioVector®

NK5449 strain" refers to a specific strain of Escherichia coli (E. coli) widely used in molecular biology and environmental science. It functions primarily as a recipient strain in laboratory experiments designed to study horizontal gene transfer—the process by which bacteria share genetic material, such as antibiotic resistance genes. Description and Characteristics Organism: Escherichia coli. Strain Designation: NK5449. Primary Use: Acts as a recipient in bacterial conjugation experiments. Key Resistance Markers: The most important characteristic of NK5449 is its intrinsic, high-level resistance to two specific antibiotics: Rifampicin (>160 mg/L) and Nalidixic Acid (>50 mg/L). This dual resistance allows researchers to easily select this strain in co-culture experiments. Plasmid Status: The strain is "plasmid-free," meaning it does not naturally contain any native plasmids, which is crucial for reliably accepting new plasmids during experiments. Supplier Information: It is cataloged by the BioVector NTCC Inc. under catalog number BioVector®-1.1437. Biosafety Level: It is typically handled under BSL-1 or BSL-2 safety levels, depending on specific experimental procedures and local laboratory safety guidelines. Usage in Research In a typical lab experiment, the NK5449 strain is mixed with a "donor" strain (e.g., E. coli HB101 containing a plasmid like RP4). By plating the mixture on agar containing all four antibiotics (kanamycin, tetracycline, rifampicin, and nalidixic acid), only the bacteria that successfully received the donor plasmid and are the resistant NK5449 strain can survive. This method is used to: Evaluate the efficiency of how plasmids transfer between bacteria. Assess how environmental factors (like microplastics or pollutants) affect the spread of antibiotic resistance. Culture and Handling Culture Medium: Standard Luria–Bertani (LB) broth or agar plates work well for growth. Selection: Appropriate concentrations of rifampicin and/or nalidixic acid must be added to the media if selection pressure is required. Temperature: Cultured aerobically at 37°C. NK5449 株系指的是大肠杆菌 (E. coli) 的一个特定菌株，广泛应用于分子生物学和环境科学领域。它主要在实验室实验中用作受体菌株，用于研究抗生素耐药基因通过接合作用进行水平转移的过程。 描述和特征 生物体： Escherichia coli (大肠杆菌)。 菌株名称： NK5449。 主要用途： 在细菌接合实验中充当受体。 关键耐药标记： NK5449 最重要的特征是其对两种特定抗生素具有固有的高水平耐药性：利福平 (>160 mg/L) 和 萘啶酸 (>50 mg/L)。这种双重耐药性使研究人员可以在共培养实验中轻松筛选该菌株。 质粒状态： 该菌株“不含质粒”，意味着其天然不含任何内源质粒，这对于在实验中可靠地接受新质粒至关重要。 供应商信息： BioVector®-1.1437。 生物安全级别： 根据具体的实验程序和当地实验室安全指南，通常在 BSL-1 或 BSL-2 安全级别下处理。 研究用途 在典型的实验室实验中，NK5449 菌株与供体菌株（例如，含有 RP4 质粒的大肠杆菌 HB101）混合。通过在含有所有四种抗生素（来自供体质粒的卡那霉素/四环素，以及来自受体 NK5449 菌株的利福平/萘啶酸）的琼脂平板上培养混合物，只有成功接收供体质粒并且是耐药 NK5449 菌株的细菌才能存活。这种方法用于： 评估质粒在细菌之间转移的效率。 评估环境因素（如微塑料或污染物）如何影响抗生素耐药性的传播。 培养和处理 培养基： 标准的 Luria–Bertani (LB) 肉汤或琼脂平板适合其生长。 筛选： 如果需要筛选压力，必须在培养基中添加适当浓度的利福平和/或萘啶酸。 温度： 在 37°C 的好氧培养箱中培养。 BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心 电话:400-800-2947 工作QQ/微信同号:1843439339 网址 www.biovector.net

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