pPROBE-gfp\(ASV\) 广宿主范围的启动子探针载体BioVector® BioVector NTCC保藏中心
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BioVector® NTCC® pPROBE-gfp\(ASV\) 广宿主范围的启动子探针载体BioVector®
ThinkingSearching The pPROBE-gfp\(ASV\) plasmid is a broad-host-range promoter-probe vector used as a transcriptional reporter system in various Gram-negative bacteria. It contains an unstable variant of the green fluorescent protein (Gfp) as a reporter gene to study real-time, dynamic gene expression. Plasmid Details Vector Name: pPROBE-gfp\(ASV\) (often referred to as pPROBE'-gfp\(ASV\))Vector Type: Broad-host-range promoter-probe vectorDeposited by: Steven Lindow (UC Davis)Bacterial Resistance: Kanamycin (50 µg/mL)Backbone Origin: pBBR1 oriV (origin of replication), allowing replication in a wide range of bacterial species.Length: Approximately 7385 base pairs (bp). Key Components Reporter Gene: gfp\(ASV\) gene.Gfp\(ASV\): This is a variant of the Green Fluorescent Protein with a short half-life (unstable). The "ASV" tag targets the protein for degradation by the host cell's lon protease, allowing for the detection of rapid changes in promoter activity.Promoter-Probe Cassette: A multicloning site (MCS) is located upstream of the gfp gene. Researchers ligate specific promoter sequences into this MCS. The expression of the Gfp reporter is then entirely dependent on the inserted promoter's activity.Transcriptional Terminators: Strong transcriptional terminators flank the reporter cassette to prevent transcription from external or vector-borne promoters from interfering with the assay. Usage and Application Function: Used to quantify gene expression driven by an inserted promoter sequence in various Gram-negative bacteria.Assay Method: Promoter activity can be quantified using flow cytometry or colony assays by measuring the fluorescence signals.Advantage: The unstable Gfp variant provides a more immediate and dynamic measure of promoter activity compared to stable Gfp variants, which accumulate over time. pPROBE-gfp\(ASV\) 质粒是一种广宿主范围的启动子探针载体，用作多种革兰氏阴性细菌中的转录报告系统。它含有一个不稳定的绿色荧光蛋白 (Gfp) 变体作为报告基因，用于研究实时、动态的基因表达。 质粒详细信息 载体名称： pPROBE-gfp\(ASV\)（通常称为 pPROBE'-gfp\(ASV\)）载体类型： 广宿主范围启动子探针载体提供者： Steven Lindow (UC Davis)细菌抗性： 卡那霉素 (50 µg/mL)骨架来源： pBBR1 oriV（复制起点），允许在多种细菌物种中复制。长度： 约 7385 个碱基对 (bp)。 主要组成部分 报告基因： gfp\(ASV\) 基因。Gfp\(ASV\)： 这是绿色荧光蛋白的一个变体，半衰期短（不稳定）。"ASV" 标签引导该蛋白被宿主细胞的 lon 蛋白酶降解，从而可以检测启动子活性的快速变化。启动子探针盒： 在 gfp 基因上游有一个多克隆位点 (MCS)。研究人员将特定的启动子序列连接到此 MCS 中。Gfp 报告基因的表达完全取决于插入的启动子的活性。转录终止子： 强的转录终止子位于报告基因盒两侧，以防止来自外部或载体上的启动子干扰检测。 用途和应用 功能： 用于量化由插入的启动子序列驱动的基因在各种革兰氏阴性细菌中的表达。检测方法： 启动子活性可以通过流式细胞术或菌落分析（通过测量荧光信号）来量化。优势： 与随时间积累的稳定 Gfp 变体相比，不稳定的 Gfp 变体提供了更即时、动态的启动子活性测量。 BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心 电话:400-800-2947 工作QQ/微信同号:1843439339 网址 www.biovector.net
Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net
ThinkingSearching The pPROBE-gfp\(ASV\) plasmid is a broad-host-range promoter-probe vector used as a transcriptional reporter system in various Gram-negative bacteria. It contains an unstable variant of the green fluorescent protein (Gfp) as a reporter gene to study real-time, dynamic gene expression. Plasmid Details Vector Name: pPROBE-gfp\(ASV\) (often referred to as pPROBE'-gfp\(ASV\))Vector Type: Broad-host-range promoter-probe vectorDeposited by: Steven Lindow (UC Davis)Bacterial Resistance: Kanamycin (50 µg/mL)Backbone Origin: pBBR1 oriV (origin of replication), allowing replication in a wide range of bacterial species.Length: Approximately 7385 base pairs (bp). Key Components Reporter Gene: gfp\(ASV\) gene.Gfp\(ASV\): This is a variant of the Green Fluorescent Protein with a short half-life (unstable). The "ASV" tag targets the protein for degradation by the host cell's lon protease, allowing for the detection of rapid changes in promoter activity.Promoter-Probe Cassette: A multicloning site (MCS) is located upstream of the gfp gene. Researchers ligate specific promoter sequences into this MCS. The expression of the Gfp reporter is then entirely dependent on the inserted promoter's activity.Transcriptional Terminators: Strong transcriptional terminators flank the reporter cassette to prevent transcription from external or vector-borne promoters from interfering with the assay. Usage and Application Function: Used to quantify gene expression driven by an inserted promoter sequence in various Gram-negative bacteria.Assay Method: Promoter activity can be quantified using flow cytometry or colony assays by measuring the fluorescence signals.Advantage: The unstable Gfp variant provides a more immediate and dynamic measure of promoter activity compared to stable Gfp variants, which accumulate over time. pPROBE-gfp\(ASV\) 质粒是一种广宿主范围的启动子探针载体，用作多种革兰氏阴性细菌中的转录报告系统。它含有一个不稳定的绿色荧光蛋白 (Gfp) 变体作为报告基因，用于研究实时、动态的基因表达。 质粒详细信息 载体名称： pPROBE-gfp\(ASV\)（通常称为 pPROBE'-gfp\(ASV\)）载体类型： 广宿主范围启动子探针载体提供者： Steven Lindow (UC Davis)细菌抗性： 卡那霉素 (50 µg/mL)骨架来源： pBBR1 oriV（复制起点），允许在多种细菌物种中复制。长度： 约 7385 个碱基对 (bp)。 主要组成部分 报告基因： gfp\(ASV\) 基因。Gfp\(ASV\)： 这是绿色荧光蛋白的一个变体，半衰期短（不稳定）。"ASV" 标签引导该蛋白被宿主细胞的 lon 蛋白酶降解，从而可以检测启动子活性的快速变化。启动子探针盒： 在 gfp 基因上游有一个多克隆位点 (MCS)。研究人员将特定的启动子序列连接到此 MCS 中。Gfp 报告基因的表达完全取决于插入的启动子的活性。转录终止子： 强的转录终止子位于报告基因盒两侧，以防止来自外部或载体上的启动子干扰检测。 用途和应用 功能： 用于量化由插入的启动子序列驱动的基因在各种革兰氏阴性细菌中的表达。检测方法： 启动子活性可以通过流式细胞术或菌落分析（通过测量荧光信号）来量化。优势： 与随时间积累的稳定 Gfp 变体相比，不稳定的 Gfp 变体提供了更即时、动态的启动子活性测量。 BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心 电话:400-800-2947 工作QQ/微信同号:1843439339 网址 www.biovector.net
Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net
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