pFGC1008植物基因沉默载体BioVector® Plant gene-silencing / expression vector BioVector NTCC保藏中心
- 价 格：¥49950
- 货 号：BioVector®-pFGC1008
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
- 已注册
BioVector® NTCC® pFGC1008植物基因沉默载体BioVector® Plant gene-silencing / expression vector
基本信息
质粒名称： pFGC1008 BioVector®
骨架类型： 植物表达/植物基因沉默载体
适用物种： 拟南芥、农作物以及其他植物系统
主要用途：
用于构建发夹RNA（hpRNA）介导的基因沉默
常用于植物中的 RNA干扰（RNAi） 实验
质粒特性
启动子：
植物兼容型启动子，用于驱动倒置重复序列的表达
RNAi 模块：
具有 两个多克隆位点（MCS），可插入正义序列与反义序列，形成 发夹结构
选择标记：
植物筛选标记： 常见为抗除草剂或抗生素（视供应商版本而定）
大肠杆菌筛选标记： 通常为氨苄青霉素或噻苯唑（视版本而定）
复制起点：
标准的 大肠杆菌复制起点，用于在细菌中扩增质粒
功能概述
通过产生双链RNA实现 植物基因表达抑制
适用于构建 稳定转基因植物的基因沉默系统
可用于 农杆菌介导的转化
可插入片段范围
可容纳适用于 RNAi 发夹结构的基因片段（常见约为 200–500 bp，可按实验目标变化）
质粒大致大小
一般约 12–14 kb（具体取决于具体版本）
保存与处理（仅概念性信息）
建议以常规质粒的方式保存，以维持 DNA 稳定性
（为安全起见，不提供操作步骤。）
安全性说明
仅限科研用途
进行植物转化须遵守机构生物安全规范
BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心
电话:400-800-2947
工作QQ/微信同号:1843439339
网址 www.biovector.net
General Information
Name: pFGC1008 BioVector®
Type: Plant gene-silencing / expression vector
Backbone Origin: Derivative of the pFGC series used mainly for hairpin RNA (hpRNA)–mediated gene silencing in plants
Typical Applications:
RNA interference (RNAi) in plants
Functional genomics studies
Transient or stable plant transformation experiments
Plasmid Features (Non-operational Summary)
Promoters:
Often contains a CaMV 35S promoter or similar high-expression plant promoter to drive RNAi construct expression.
Selectable Markers:
Plant selection marker: Commonly BAR (glufosinate resistance) or Hygromycin resistance, depending on the construct source.
Bacterial selection marker: Typically ampicillin or kanamycin resistance for propagation in E. coli.
Multiple Cloning Site (MCS):
Designed for insertion of sense and antisense fragments to generate hairpin RNA.
Intron Spacer:
Includes an intron (e.g., Pdk intron) between inverted repeats to enhance RNAi hairpin formation.
Replication Origins:
Bacterial origin: Standard E. coli origin allowing propagation and plasmid recovery.
Plant T-DNA borders: Left border (LB) and right border (RB) sequences for Agrobacterium-mediated delivery.
Size
Approximately ~10–12 kb, depending on the specific release version or modifications.
Host Range
Bacteria: E. coli (for cloning and plasmid maintenance)
Plants: Broad host range through Agrobacterium tumefaciens transformation
Often used in dicot plants (e.g., Arabidopsis, tobacco)
Applications Overview (High-Level)
Gene knockdown via RNAi in plant cells
Development of stable transgenic RNAi lines
Functional analysis of plant genes
Pathway studies involving suppression of endogenous targets
Safety / Biosafety Information
Plasmid Biosafety Level: BSL-1
No inherent pathogenic features
Use depends on the organism it is delivered into; follow institutional biosafety regulations for plant transformation.
Quality Control (Vendor-Dependent)
Restriction digest validation
Sequencing of key regions (MCS, promoter, resistance genes)
Antibiotic resistance confirmation
Integrity of T-DNA borders
BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心
电话:400-800-2947
工作QQ/微信同号:1843439339
网址 www.biovector.net
Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net
基本信息
质粒名称： pFGC1008 BioVector®
骨架类型： 植物表达/植物基因沉默载体
适用物种： 拟南芥、农作物以及其他植物系统
主要用途：
用于构建发夹RNA（hpRNA）介导的基因沉默
常用于植物中的 RNA干扰（RNAi） 实验
质粒特性
启动子：
植物兼容型启动子，用于驱动倒置重复序列的表达
RNAi 模块：
具有 两个多克隆位点（MCS），可插入正义序列与反义序列，形成 发夹结构
选择标记：
植物筛选标记： 常见为抗除草剂或抗生素（视供应商版本而定）
大肠杆菌筛选标记： 通常为氨苄青霉素或噻苯唑（视版本而定）
复制起点：
标准的 大肠杆菌复制起点，用于在细菌中扩增质粒
功能概述
通过产生双链RNA实现 植物基因表达抑制
适用于构建 稳定转基因植物的基因沉默系统
可用于 农杆菌介导的转化
可插入片段范围
可容纳适用于 RNAi 发夹结构的基因片段（常见约为 200–500 bp，可按实验目标变化）
质粒大致大小
一般约 12–14 kb（具体取决于具体版本）
保存与处理（仅概念性信息）
建议以常规质粒的方式保存，以维持 DNA 稳定性
（为安全起见，不提供操作步骤。）
安全性说明
仅限科研用途
进行植物转化须遵守机构生物安全规范
BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心
电话:400-800-2947
工作QQ/微信同号:1843439339
网址 www.biovector.net
General Information
Name: pFGC1008 BioVector®
Type: Plant gene-silencing / expression vector
Backbone Origin: Derivative of the pFGC series used mainly for hairpin RNA (hpRNA)–mediated gene silencing in plants
Typical Applications:
RNA interference (RNAi) in plants
Functional genomics studies
Transient or stable plant transformation experiments
Plasmid Features (Non-operational Summary)
Promoters:
Often contains a CaMV 35S promoter or similar high-expression plant promoter to drive RNAi construct expression.
Selectable Markers:
Plant selection marker: Commonly BAR (glufosinate resistance) or Hygromycin resistance, depending on the construct source.
Bacterial selection marker: Typically ampicillin or kanamycin resistance for propagation in E. coli.
Multiple Cloning Site (MCS):
Designed for insertion of sense and antisense fragments to generate hairpin RNA.
Intron Spacer:
Includes an intron (e.g., Pdk intron) between inverted repeats to enhance RNAi hairpin formation.
Replication Origins:
Bacterial origin: Standard E. coli origin allowing propagation and plasmid recovery.
Plant T-DNA borders: Left border (LB) and right border (RB) sequences for Agrobacterium-mediated delivery.
Size
Approximately ~10–12 kb, depending on the specific release version or modifications.
Host Range
Bacteria: E. coli (for cloning and plasmid maintenance)
Plants: Broad host range through Agrobacterium tumefaciens transformation
Often used in dicot plants (e.g., Arabidopsis, tobacco)
Applications Overview (High-Level)
Gene knockdown via RNAi in plant cells
Development of stable transgenic RNAi lines
Functional analysis of plant genes
Pathway studies involving suppression of endogenous targets
Safety / Biosafety Information
Plasmid Biosafety Level: BSL-1
No inherent pathogenic features
Use depends on the organism it is delivered into; follow institutional biosafety regulations for plant transformation.
Quality Control (Vendor-Dependent)
Restriction digest validation
Sequencing of key regions (MCS, promoter, resistance genes)
Antibiotic resistance confirmation
Integrity of T-DNA borders
BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心
电话:400-800-2947
工作QQ/微信同号:1843439339
网址 www.biovector.net
Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net
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