β2155大肠杆菌营养缺陷型细菌偶联实验供体菌株BioVector® Conjugation-deficient donor strain BioVector NTCC保藏中心
- 价 格:¥49950
- 货 号:BioVector®-Escherichia coli β2155
- 产 地:北京
- BioVector NTCC典型培养物保藏中心
- 联系人:Dr.Xu, Biovector NTCC Inc.
电话:400-800-2947 工作微信:1843439339 (QQ同号)
邮件:Biovector@163.com
手机:18901268599
地址:北京
- 已注册
BioVector® NTCC® β2155大肠杆菌营养缺陷型细菌偶联实验供体菌株BioVector® Conjugation-deficient donor strain
基本信息
名称: Escherichia coli β2155 NTCC®
类别: 实验室工程菌株
常用用途: DNA 克隆、基因构建、细菌偶联(conjugation)
特点: 为无乳糖可发酵菌株(Lac–),广泛用于分子生物学研究
基因型(高层级,不含可用于操作的细节)
β2155 为一个经工程改造的实验室菌株,一般具有:
缺失一部分代谢路径,需要在培养基中外源补充特定营养因子
含有支持细菌偶联的遗传元件
含有便于筛选的标记基因
(具体基因型可从BioVector NTCC保藏中心获取。)
主要应用方向
细菌偶联实验(bacterial conjugation):常作为供体菌株用于将质粒或基因构建转移给其他细菌
分子克隆与载体构建
遗传工程研究
研究细菌间水平基因转移机制
生长与基本特性(非操作性概述)
生长需求中常包含某些外源性营养因子
属于需辅助因子的“营养缺陷型”菌株
生长速度低于常规 E. coli 野生型
质量控制(供应商通常提供)
纯度鉴定
菌株基因型验证
抗性标记验证
无污染检测(如霉菌、其他细菌)
生物安全信息
风险等级: 通常为 BSL-1(依实验类型与国家法规可能不同)
用于基础科研,无致病性,不适用于临床或环境释放
使用时应遵守标准微生物安全规范
BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心
电话:400-800-2947
工作QQ/微信同号:1843439339
网址 www.biovector.net
General Information
Name: Escherichia coli β2155 NTCC®
Genotype Type: Conjugation-deficient donor strain
Key Feature: dapA mutant, requires exogenous diaminopimelic acid (DAP) for growth
Primary Use:
Mating-based DNA transfer (e.g., conjugation) into Gram-negative bacteria
mobilization of plasmids carrying RP4 oriT
safe donor strain because DAP auxotrophy prevents environmental persistence
Genotype (High-Level)
ΔdapA — DAP auxotroph
RP4 tra functions integrated into chromosome
recA mutation in many versions (reduces recombination)
Exact genotype varies by supplier; refer to the specific repository for official characterization.
Phenotypic Features
DAP Auxotrophy: Cannot grow without supplemented diaminopimelic acid
Efficient Donor Capability: Commonly used for mobilizing plasmids into diverse bacteria
Non-pathogenic Laboratory Strain
Biosafety
Risk Group: RG1 (typical lab E. coli K-12 derivatives), though local regulations may vary
Safe for standard molecular biology use
DAP requirement limits survival outside controlled environments
Applications
Conjugative plasmid transfer into:
Vibrio species
Pseudomonas
Shewanella
Many other Gram-negative bacteria
Used in microbial genetics and synthetic biology workflows
Quality Control (from repositories)
Genotype verification
Antibiotic resistance verification (varies with plasmid carried at distribution time)
Growth dependence on DAP
Standard sterility QC
Notes
This strain is frequently used with mobilizable plasmids that contain:
oriT (RP4 origin of transfer)
but lacks the ability to self-transfer without donor machinery.
BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心
电话:400-800-2947
工作QQ/微信同号:1843439339
网址 www.biovector.net
Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net
基本信息
名称: Escherichia coli β2155 NTCC®
类别: 实验室工程菌株
常用用途: DNA 克隆、基因构建、细菌偶联(conjugation)
特点: 为无乳糖可发酵菌株(Lac–),广泛用于分子生物学研究
基因型(高层级,不含可用于操作的细节)
β2155 为一个经工程改造的实验室菌株,一般具有:
缺失一部分代谢路径,需要在培养基中外源补充特定营养因子
含有支持细菌偶联的遗传元件
含有便于筛选的标记基因
(具体基因型可从BioVector NTCC保藏中心获取。)
主要应用方向
细菌偶联实验(bacterial conjugation):常作为供体菌株用于将质粒或基因构建转移给其他细菌
分子克隆与载体构建
遗传工程研究
研究细菌间水平基因转移机制
生长与基本特性(非操作性概述)
生长需求中常包含某些外源性营养因子
属于需辅助因子的“营养缺陷型”菌株
生长速度低于常规 E. coli 野生型
质量控制(供应商通常提供)
纯度鉴定
菌株基因型验证
抗性标记验证
无污染检测(如霉菌、其他细菌)
生物安全信息
风险等级: 通常为 BSL-1(依实验类型与国家法规可能不同)
用于基础科研,无致病性,不适用于临床或环境释放
使用时应遵守标准微生物安全规范
BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心
电话:400-800-2947
工作QQ/微信同号:1843439339
网址 www.biovector.net
General Information
Name: Escherichia coli β2155 NTCC®
Genotype Type: Conjugation-deficient donor strain
Key Feature: dapA mutant, requires exogenous diaminopimelic acid (DAP) for growth
Primary Use:
Mating-based DNA transfer (e.g., conjugation) into Gram-negative bacteria
mobilization of plasmids carrying RP4 oriT
safe donor strain because DAP auxotrophy prevents environmental persistence
Genotype (High-Level)
ΔdapA — DAP auxotroph
RP4 tra functions integrated into chromosome
recA mutation in many versions (reduces recombination)
Exact genotype varies by supplier; refer to the specific repository for official characterization.
Phenotypic Features
DAP Auxotrophy: Cannot grow without supplemented diaminopimelic acid
Efficient Donor Capability: Commonly used for mobilizing plasmids into diverse bacteria
Non-pathogenic Laboratory Strain
Biosafety
Risk Group: RG1 (typical lab E. coli K-12 derivatives), though local regulations may vary
Safe for standard molecular biology use
DAP requirement limits survival outside controlled environments
Applications
Conjugative plasmid transfer into:
Vibrio species
Pseudomonas
Shewanella
Many other Gram-negative bacteria
Used in microbial genetics and synthetic biology workflows
Quality Control (from repositories)
Genotype verification
Antibiotic resistance verification (varies with plasmid carried at distribution time)
Growth dependence on DAP
Standard sterility QC
Notes
This strain is frequently used with mobilizable plasmids that contain:
oriT (RP4 origin of transfer)
but lacks the ability to self-transfer without donor machinery.
BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心
电话:400-800-2947
工作QQ/微信同号:1843439339
网址 www.biovector.net
Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net
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