EGFP-Plekhh1真核表达质粒BioVector® plasmid BioVector NTCC保藏中心
- 价 格：¥39985
- 货 号：BioVector®-EGFP-Plekhh1
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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BioVector® NTCC® EGFP-Plekhh1真核表达质粒BioVector® plasmid
General Information
Plasmid Name: EGFP-Plekhh1 BioVector®
Type: Mammalian expression plasmid
Insert: Plekhh1 (Pleckstrin homology domain–containing family H member 1)
Tag: EGFP fused to Plekhh1, usually at the N-terminus for live-cell visualization
Typical Purpose:
Investigation of Plekhh1 localization
Cytoskeletal or membrane signaling studies
Overexpression studies in mammalian cells
Backbone Features (High-Level)
(General characteristics of common EGFP expression vectors; details may vary depending on the exact construct source.)
Promoter: Strong mammalian promoter (commonly CMV or similar) for high-level expression
Fluorescent Reporter: Enhanced Green Fluorescent Protein (EGFP) for visualization
Polyadenylation Signal: Typically SV40 or similar
Multiple Cloning Site (MCS): Used to insert the Plekhh1 ORF
Origin of Replication: High-copy bacterial origin (e.g., pUC ori) for propagation in E. coli
Antibiotic Selection (Bacteria): Usually ampicillin or kanamycin
Mammalian Selection (if included): Some variants include markers like neomycin, puromycin, or none (depends on specific construct source)
Insert Information: Plekhh1
Gene: Pleckstrin homology domain-containing family H member 1
Protein Domains:
PH domain
Coiled-coil regions
Possible Rho-GTPase–associated domains
Biological Roles (research context):
Cytoskeletal organization
Apical/planar cell polarity
Signal transduction pathways
(These are research associations, not functional claims.)
Expression Characteristics
Designed for fluorescent visualization of Plekhh1 dynamics
Localizes depending on Plekhh1’s natural context (often associated with membrane or cytoskeletal complexes)
Suitable for imaging using standard GFP filter sets
Applications
Subcellular localization studies
Protein–protein interaction screening (non-procedural)
Live-cell imaging of Plekhh1 distribution
Exploratory research on PH-domain proteins
Storage & Handling (High-Level)
DNA is generally stored frozen by vendors
Propagated in standard cloning strains of E. coli
Follow vendor or institutional biosafety rules for recombinant DNA
基本信息
名称： BioVector® EGFP-Plekhh1 表达质粒
类型： 真核表达载体
标签蛋白： EGFP（增强型绿色荧光蛋白）
插入基因： Plekhh1（Pleckstrin Homology Domain Containing Family H Member 1）
用途： 在哺乳动物细胞中表达带有 EGFP 标签的 Plekhh1 蛋白，用于定位、蛋白相互作用研究、细胞动力学观察等。
载体特征（高层次信息）
启动子： 常见 CMV 或同类强启动子（取决于具体构建来源）
报告基因： EGFP 与 Plekhh1 融合
抗性标记（细胞选择）： 常见为嘌呤霉素或新霉素（以供应商说明为准）
抗性标记（细菌）： 典型为氨苄青霉素
复制子： 常见 ColE1 类型（取决于构建背景）
应用方向
研究 Plekhh1 在细胞骨架动力学中的作用
观察 Plekhh1 的亚细胞定位
蛋白质–蛋白质相互作用研究
分子机制研究、信号通路分析
荧光示踪实验
荧光特点（EGFP 标签）
激发峰：约 488 nm
发射峰：约 509 nm
可用于实时成像、共聚焦成像、筛选 EGFP 阳性细胞群体
储存与注意事项（无操作步骤）
质粒应在供应商要求的条件下保存（通常为超低温）
使用前请核对序列结构、标签位置、选择标记等
实验中应遵循生物安全规范并在适当等级实验室内操作
质量控制
序列验证（Sanger 或 NGS）
质粒纯度检测
抗性标记验证
插入片段完整性检测
BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心
电话:400-800-2947
工作QQ/微信同号:1843439339
网址 www.biovector.net
Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net
General Information
Plasmid Name: EGFP-Plekhh1 BioVector®
Type: Mammalian expression plasmid
Insert: Plekhh1 (Pleckstrin homology domain–containing family H member 1)
Tag: EGFP fused to Plekhh1, usually at the N-terminus for live-cell visualization
Typical Purpose:
Investigation of Plekhh1 localization
Cytoskeletal or membrane signaling studies
Overexpression studies in mammalian cells
Backbone Features (High-Level)
(General characteristics of common EGFP expression vectors; details may vary depending on the exact construct source.)
Promoter: Strong mammalian promoter (commonly CMV or similar) for high-level expression
Fluorescent Reporter: Enhanced Green Fluorescent Protein (EGFP) for visualization
Polyadenylation Signal: Typically SV40 or similar
Multiple Cloning Site (MCS): Used to insert the Plekhh1 ORF
Origin of Replication: High-copy bacterial origin (e.g., pUC ori) for propagation in E. coli
Antibiotic Selection (Bacteria): Usually ampicillin or kanamycin
Mammalian Selection (if included): Some variants include markers like neomycin, puromycin, or none (depends on specific construct source)
Insert Information: Plekhh1
Gene: Pleckstrin homology domain-containing family H member 1
Protein Domains:
PH domain
Coiled-coil regions
Possible Rho-GTPase–associated domains
Biological Roles (research context):
Cytoskeletal organization
Apical/planar cell polarity
Signal transduction pathways
(These are research associations, not functional claims.)
Expression Characteristics
Designed for fluorescent visualization of Plekhh1 dynamics
Localizes depending on Plekhh1’s natural context (often associated with membrane or cytoskeletal complexes)
Suitable for imaging using standard GFP filter sets
Applications
Subcellular localization studies
Protein–protein interaction screening (non-procedural)
Live-cell imaging of Plekhh1 distribution
Exploratory research on PH-domain proteins
Storage & Handling (High-Level)
DNA is generally stored frozen by vendors
Propagated in standard cloning strains of E. coli
Follow vendor or institutional biosafety rules for recombinant DNA
基本信息
名称： BioVector® EGFP-Plekhh1 表达质粒
类型： 真核表达载体
标签蛋白： EGFP（增强型绿色荧光蛋白）
插入基因： Plekhh1（Pleckstrin Homology Domain Containing Family H Member 1）
用途： 在哺乳动物细胞中表达带有 EGFP 标签的 Plekhh1 蛋白，用于定位、蛋白相互作用研究、细胞动力学观察等。
载体特征（高层次信息）
启动子： 常见 CMV 或同类强启动子（取决于具体构建来源）
报告基因： EGFP 与 Plekhh1 融合
抗性标记（细胞选择）： 常见为嘌呤霉素或新霉素（以供应商说明为准）
抗性标记（细菌）： 典型为氨苄青霉素
复制子： 常见 ColE1 类型（取决于构建背景）
应用方向
研究 Plekhh1 在细胞骨架动力学中的作用
观察 Plekhh1 的亚细胞定位
蛋白质–蛋白质相互作用研究
分子机制研究、信号通路分析
荧光示踪实验
荧光特点（EGFP 标签）
激发峰：约 488 nm
发射峰：约 509 nm
可用于实时成像、共聚焦成像、筛选 EGFP 阳性细胞群体
储存与注意事项（无操作步骤）
质粒应在供应商要求的条件下保存（通常为超低温）
使用前请核对序列结构、标签位置、选择标记等
实验中应遵循生物安全规范并在适当等级实验室内操作
质量控制
序列验证（Sanger 或 NGS）
质粒纯度检测
抗性标记验证
插入片段完整性检测
BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心
电话:400-800-2947
工作QQ/微信同号:1843439339
网址 www.biovector.net
Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net
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