FGY217酿酒酵母（Saccharomyces cerevisiae）PEP4基因缺失单倍体菌株 BioVector NTCC保藏中心
- 价 格：¥89950
- 货 号：BioVector®-FGY217
- 产 地：北京
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- 联系人：Dr.Xu, Biovector NTCC Inc.
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邮件：Biovector@163.com
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BioVector® NTCC® FGY217酿酒酵母（Saccharomyces cerevisiae）PEP4基因缺失单倍体菌株
BioVector® FGY217菌株是酿酒酵母（Saccharomyces cerevisiae）的一种单倍体菌株，常用于分子生物学中的蛋白质表达研究。它特别值得注意，因为该菌株缺失了PEP4基因，因此可用于过量表达那些通常会被液泡中的蛋白酶降解的蛋白质。
遗传背景
根据多个来源，FGY217菌株的具体基因型为MATa, ura3-52, lys2Δ201, pep4Δ。
MATa：指定酵母的交配型。
ura3-52：一个突变，使该酵母菌株对尿嘧啶呈营养缺陷型，这使得研究人员能够利用尿嘧啶缺乏作为选择标记。
lys2Δ201：lys2基因的一个突变，导致赖氨酸缺陷，也可用于筛选。
pep4Δ：PEP4基因的一个缺失突变。该基因编码一种液泡内肽酶。它的缺失会使这种蛋白酶失活，并且重要的是，还会导致其他液泡水解酶的总体水平降低，从而防止不必要的蛋白质降解。
研究应用
FGY217菌株在以下应用中非常有价值：
膜蛋白表达：它常用于异源表达真核膜蛋白，例如钾离子通道，否则这些蛋白质可能难以大量生产。
优化研究：该菌株常用于高通量优化方案中，以生产蛋白质。研究表明，与其他菌株相比，FGY217中的蛋白质表达水平通常更高。
GFP融合研究：它与生产绿色荧光蛋白（GFP）融合蛋白的系统兼容，使研究人员能够追踪蛋白质定位并监测表达水平。
过表达筛选：FGY217可用于通过同源重组插入基因，从而快速筛选和评估各种蛋白质的过表达潜力。
The BioVector® FGY217 strain is a haploid yeast strain of Saccharomyces cerevisiae, commonly used in molecular biology for protein expression studies. It is particularly notable because it has a deletion of the PEP4 gene, which makes it useful for overexpressing proteins that would normally be degraded by proteases in the vacuole.
Genetic background
According to multiple sources, the specific genetic makeup of the FGY217 strain is MATa, ura3-52, lys2Δ201, pep4Δ.
MATa: Specifies the mating type of the yeast.
ura3-52: A mutation that makes the yeast strain auxotrophic for uracil, allowing researchers to use uracil deficiency as a selection marker.
lys2Δ201: A mutation in the lys2 gene causing a lysine deficiency, which can also be used for selection.
pep4Δ: A deletion mutation in the PEP4 gene. This gene encodes a vacuolar endopeptidase. Its deletion inactivates this protease and, importantly, leads to the overall reduction of other vacuolar hydrolases, which prevents unwanted protein degradation.
Research applications
The FGY217 strain is highly valued for the following applications:
Membrane protein expression: It is frequently used for the heterologous expression of eukaryotic membrane proteins, such as potassium channels, which might otherwise be difficult to produce in large quantities.
Optimization studies: The strain is often used in high-throughput optimization schemes for producing proteins. Studies have shown that protein expression levels are often higher in FGY217 compared to other strains.
GFP-fusion studies: It is compatible with systems for producing green fluorescent protein (GFP)-fusion proteins, allowing researchers to track protein localization and monitor expression levels.
Overexpression screening: FGY217 is used for rapidly screening and evaluating the potential for overexpression of various proteins by inserting genes using homologous recombination.
Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net
BioVector® FGY217菌株是酿酒酵母（Saccharomyces cerevisiae）的一种单倍体菌株，常用于分子生物学中的蛋白质表达研究。它特别值得注意，因为该菌株缺失了PEP4基因，因此可用于过量表达那些通常会被液泡中的蛋白酶降解的蛋白质。
遗传背景
根据多个来源，FGY217菌株的具体基因型为MATa, ura3-52, lys2Δ201, pep4Δ。
MATa：指定酵母的交配型。
ura3-52：一个突变，使该酵母菌株对尿嘧啶呈营养缺陷型，这使得研究人员能够利用尿嘧啶缺乏作为选择标记。
lys2Δ201：lys2基因的一个突变，导致赖氨酸缺陷，也可用于筛选。
pep4Δ：PEP4基因的一个缺失突变。该基因编码一种液泡内肽酶。它的缺失会使这种蛋白酶失活，并且重要的是，还会导致其他液泡水解酶的总体水平降低，从而防止不必要的蛋白质降解。
研究应用
FGY217菌株在以下应用中非常有价值：
膜蛋白表达：它常用于异源表达真核膜蛋白，例如钾离子通道，否则这些蛋白质可能难以大量生产。
优化研究：该菌株常用于高通量优化方案中，以生产蛋白质。研究表明，与其他菌株相比，FGY217中的蛋白质表达水平通常更高。
GFP融合研究：它与生产绿色荧光蛋白（GFP）融合蛋白的系统兼容，使研究人员能够追踪蛋白质定位并监测表达水平。
过表达筛选：FGY217可用于通过同源重组插入基因，从而快速筛选和评估各种蛋白质的过表达潜力。
The BioVector® FGY217 strain is a haploid yeast strain of Saccharomyces cerevisiae, commonly used in molecular biology for protein expression studies. It is particularly notable because it has a deletion of the PEP4 gene, which makes it useful for overexpressing proteins that would normally be degraded by proteases in the vacuole.
Genetic background
According to multiple sources, the specific genetic makeup of the FGY217 strain is MATa, ura3-52, lys2Δ201, pep4Δ.
MATa: Specifies the mating type of the yeast.
ura3-52: A mutation that makes the yeast strain auxotrophic for uracil, allowing researchers to use uracil deficiency as a selection marker.
lys2Δ201: A mutation in the lys2 gene causing a lysine deficiency, which can also be used for selection.
pep4Δ: A deletion mutation in the PEP4 gene. This gene encodes a vacuolar endopeptidase. Its deletion inactivates this protease and, importantly, leads to the overall reduction of other vacuolar hydrolases, which prevents unwanted protein degradation.
Research applications
The FGY217 strain is highly valued for the following applications:
Membrane protein expression: It is frequently used for the heterologous expression of eukaryotic membrane proteins, such as potassium channels, which might otherwise be difficult to produce in large quantities.
Optimization studies: The strain is often used in high-throughput optimization schemes for producing proteins. Studies have shown that protein expression levels are often higher in FGY217 compared to other strains.
GFP-fusion studies: It is compatible with systems for producing green fluorescent protein (GFP)-fusion proteins, allowing researchers to track protein localization and monitor expression levels.
Overexpression screening: FGY217 is used for rapidly screening and evaluating the potential for overexpression of various proteins by inserting genes using homologous recombination.
Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net
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