CHON-001永生化的人类软骨细胞系immortalized human chondrocyte cell line BioVector NTCC典型培养物保藏中心 BioVector NTCC保藏中心
- 价 格：¥99850
- 货 号：BioVector®-CHON-001
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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BioVector® NTCC® CHON-001永生化的人类软骨细胞系immortalized human chondrocyte cell line BioVector NTCC典型培养物保藏中心
The NTCC® CHON-001 cell line is an immortalized human chondrocyte cell line developed for use in research related to cartilage biology and disease, particularly osteoarthritis.
Key characteristics
Origin: Isolated in 2001 from the cartilage of an 18-week-old female human fetus.
Immortalization: The primary chondrocytes were immortalized using a retrovirus containing the human telomerase reverse transcriptase (hTERT) gene.
Morphology: The cells exhibit a fibroblast-like morphology.
Karyology: It is a diploid cell line with a stable karyology. It has a modal chromosome number of 46 in 93% of the cells examined.
Culture: CHON-001 cells are typically cultured in Dulbecco's Modified Eagle Medium (DMEM) supplemented with fetal bovine serum (FBS) and other additives. They can also be cultured in 3D structures like alginate beads to promote a more chondrocyte-like phenotype.
Response to stimuli: The cell line is responsive to inflammatory stimuli like interleukin-1β (IL-1β), making it a valuable in vitro model for studying osteoarthritis.
Applications
Osteoarthritis (OA) research: CHON-001 is used to study the mechanisms of inflammation and cartilage degradation involved in OA.
Drug development: The cell line serves as a tool for testing potential therapeutic agents for cartilage repair and OA treatment.
Cartilage biology: It is used to investigate chondrocyte signaling pathways, gene expression, and matrix synthesis.
Chondrogenesis: Used to study the process of cartilage formation and maturation.
CHON-001细胞系是一种永生化的人类软骨细胞系，专门用于软骨生物学和疾病（尤其是骨关节炎）相关的研究。
主要特征
来源：2001年，研究人员从一名18周大女性人类胎儿的软骨中分离并建立了该细胞系。
永生化：利用含有端粒酶逆转录酶（hTERT）基因的逆转录病毒，将原代软骨细胞永生化。
形态：细胞呈现成纤维细胞样形态。
染色体核型：这是一个稳定的二倍体细胞系。经检查，93%的细胞染色体众数为46条。
培养：CHON-001细胞通常在添加了胎牛血清（FBS）和其他添加剂的杜氏改良伊格尔培养基（DMEM）中培养。为了促进更具软骨细胞的表型，也可以在海藻酸钠凝胶珠等三维结构中培养。
对刺激的反应：该细胞系对白细胞介素-1β（IL-1β）等炎症刺激有反应，使其成为研究骨关节炎的宝贵体外模型。
应用
骨关节炎（OA）研究：CHON-001细胞被用于研究与OA相关的炎症和软骨退化机制。
药物开发：该细胞系是测试用于软骨修复和OA治疗的潜在药物的工具。
软骨生物学：用于研究软骨细胞的信号通路、基因表达和基质合成。
软骨形成：用于研究软骨形成和成熟的过程。
Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net
The NTCC® CHON-001 cell line is an immortalized human chondrocyte cell line developed for use in research related to cartilage biology and disease, particularly osteoarthritis.
Key characteristics
Origin: Isolated in 2001 from the cartilage of an 18-week-old female human fetus.
Immortalization: The primary chondrocytes were immortalized using a retrovirus containing the human telomerase reverse transcriptase (hTERT) gene.
Morphology: The cells exhibit a fibroblast-like morphology.
Karyology: It is a diploid cell line with a stable karyology. It has a modal chromosome number of 46 in 93% of the cells examined.
Culture: CHON-001 cells are typically cultured in Dulbecco's Modified Eagle Medium (DMEM) supplemented with fetal bovine serum (FBS) and other additives. They can also be cultured in 3D structures like alginate beads to promote a more chondrocyte-like phenotype.
Response to stimuli: The cell line is responsive to inflammatory stimuli like interleukin-1β (IL-1β), making it a valuable in vitro model for studying osteoarthritis.
Applications
Osteoarthritis (OA) research: CHON-001 is used to study the mechanisms of inflammation and cartilage degradation involved in OA.
Drug development: The cell line serves as a tool for testing potential therapeutic agents for cartilage repair and OA treatment.
Cartilage biology: It is used to investigate chondrocyte signaling pathways, gene expression, and matrix synthesis.
Chondrogenesis: Used to study the process of cartilage formation and maturation.
CHON-001细胞系是一种永生化的人类软骨细胞系，专门用于软骨生物学和疾病（尤其是骨关节炎）相关的研究。
主要特征
来源：2001年，研究人员从一名18周大女性人类胎儿的软骨中分离并建立了该细胞系。
永生化：利用含有端粒酶逆转录酶（hTERT）基因的逆转录病毒，将原代软骨细胞永生化。
形态：细胞呈现成纤维细胞样形态。
染色体核型：这是一个稳定的二倍体细胞系。经检查，93%的细胞染色体众数为46条。
培养：CHON-001细胞通常在添加了胎牛血清（FBS）和其他添加剂的杜氏改良伊格尔培养基（DMEM）中培养。为了促进更具软骨细胞的表型，也可以在海藻酸钠凝胶珠等三维结构中培养。
对刺激的反应：该细胞系对白细胞介素-1β（IL-1β）等炎症刺激有反应，使其成为研究骨关节炎的宝贵体外模型。
应用
骨关节炎（OA）研究：CHON-001细胞被用于研究与OA相关的炎症和软骨退化机制。
药物开发：该细胞系是测试用于软骨修复和OA治疗的潜在药物的工具。
软骨生物学：用于研究软骨细胞的信号通路、基因表达和基质合成。
软骨形成：用于研究软骨形成和成熟的过程。
Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net
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