B16/ADR BioVector®小鼠黑色素瘤阿霉素耐药细胞株 BioVector NTCC保藏中心
- 价 格：¥599850
- 货 号：BioVector®-B16/ADR
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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BioVector® NTCC® B16/ADR BioVector®小鼠黑色素瘤阿霉素耐药细胞株
NTCC® B16/ADR cell line is a adriamycin (ADR)-resistant sublines of B16 mouse melanoma cell line.
The B16 cell line is a widely used murine model derived from melanoma tumors in C57BL/6 mice. This line is extensively employed in research due to its ability to form melanotic tumors that closely resemble human melanoma in terms of growth characteristics and metastatic potential. The cell line exists in various subtypes, such as B16-F0, B16-F1, and B16-F10, with each subtype demonstrating varying degrees of metastatic capability; for instance, B16-F10 is highly metastatic compared to B16-F0. These variations allow researchers to select an appropriate model based on the specific requirements of their studies concerning tumor aggressiveness and metastasis.
B16 cells are instrumental in understanding the molecular and cellular mechanisms of melanoma progression and testing anti-cancer therapies. Their melanin-producing ability makes them particularly useful for studies on melanogenesis and its regulation. Furthermore, the B16 cell line serves as an essential tool for vaccine development and immunotherapy experiments, offering insights into tumor-immune system interactions and the efficacy of immunomodulatory agents. The adaptability of these cells to various in vivo and in vitro environments underscores their significance in translational and preclinical research aimed at melanoma treatment and prevention.
Organism Mouse
Tissue Skin
Disease Mouse melanoma
Gender Male
Morphology Mixture of spindle-shaped and epithelial-like cells
Growth properties Adherent
Tumorigenic Yes
Products Melanin
Culture Medium NTCC® EMEM, w: 0.20 microgram/ml ADR, w: 2 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: EBSS, w: 1 mM Sodium pyruvate, w: NEAA (NTCC®820100c)
Medium supplements Supplement the medium with 10% FBS
Passaging solution Accutase
Subculturing Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Split ratio 1:4 to 1:8
Fluid renewal 2 to 3 times per week
Image
Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net
NTCC® B16/ADR cell line is a adriamycin (ADR)-resistant sublines of B16 mouse melanoma cell line.
The B16 cell line is a widely used murine model derived from melanoma tumors in C57BL/6 mice. This line is extensively employed in research due to its ability to form melanotic tumors that closely resemble human melanoma in terms of growth characteristics and metastatic potential. The cell line exists in various subtypes, such as B16-F0, B16-F1, and B16-F10, with each subtype demonstrating varying degrees of metastatic capability; for instance, B16-F10 is highly metastatic compared to B16-F0. These variations allow researchers to select an appropriate model based on the specific requirements of their studies concerning tumor aggressiveness and metastasis.
B16 cells are instrumental in understanding the molecular and cellular mechanisms of melanoma progression and testing anti-cancer therapies. Their melanin-producing ability makes them particularly useful for studies on melanogenesis and its regulation. Furthermore, the B16 cell line serves as an essential tool for vaccine development and immunotherapy experiments, offering insights into tumor-immune system interactions and the efficacy of immunomodulatory agents. The adaptability of these cells to various in vivo and in vitro environments underscores their significance in translational and preclinical research aimed at melanoma treatment and prevention.
Organism Mouse
Tissue Skin
Disease Mouse melanoma
Gender Male
Morphology Mixture of spindle-shaped and epithelial-like cells
Growth properties Adherent
Tumorigenic Yes
Products Melanin
Culture Medium NTCC® EMEM, w: 0.20 microgram/ml ADR, w: 2 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: EBSS, w: 1 mM Sodium pyruvate, w: NEAA (NTCC®820100c)
Medium supplements Supplement the medium with 10% FBS
Passaging solution Accutase
Subculturing Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Split ratio 1:4 to 1:8
Fluid renewal 2 to 3 times per week
Image
Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net
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