AAVS1-tdTomato人AAVS1靶向荧光表达质粒载体BioVector® plasmid vector BioVector NTCC保藏中心
- 价 格:¥39850
- 货 号:BioVector®-AAVS1-tdTomato
- 产 地:北京
- BioVector NTCC典型培养物保藏中心
- 联系人:Dr.Xu, Biovector NTCC Inc.
电话:400-800-2947 工作微信:1843439339 (QQ同号)
邮件:Biovector@163.com
手机:18901268599
地址:北京
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BioVector® NTCC® AAVS1-tdTomato人AAVS1靶向荧光表达质粒载体BioVector® plasmid vector
Human AAVS1 targeting vector for knockin of a CAGGS promoter-driven tdTomato cassette (cloned between AgeI and PacI). Targeted cells will be puromycin resistant.
Backbone
Vector backbonepUC57
Vector typeMammalian Expression, AAV ; S1 knockin donor vector
Selectable markersPuromycin
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Temperature30°C
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Gene/Insert
Gene/Insert nametdTomato
Insert Size (bp)1431
PromoterCAGGS
Cloning Information
Cloning methodRestriction Enzyme
5′ cloning siteAgeI (not destroyed)
3′ cloning sitePacI (not destroyed)
5′ sequencing primerggcggctctagagcctctgctaacc
3′ sequencing primerttggcagagggaaaaagatctcagtgg
Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net
Human AAVS1 targeting vector for knockin of a CAGGS promoter-driven tdTomato cassette (cloned between AgeI and PacI). Targeted cells will be puromycin resistant.
Backbone
Vector backbonepUC57
Vector typeMammalian Expression, AAV ; S1 knockin donor vector
Selectable markersPuromycin
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Temperature30°C
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Gene/Insert
Gene/Insert nametdTomato
Insert Size (bp)1431
PromoterCAGGS
Cloning Information
Cloning methodRestriction Enzyme
5′ cloning siteAgeI (not destroyed)
3′ cloning sitePacI (not destroyed)
5′ sequencing primerggcggctctagagcctctgctaacc
3′ sequencing primerttggcagagggaaaaagatctcagtgg
Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net
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