pSC189细菌转座子递送载体BioVector® C9转座酶质粒 BioVector NTCC保藏中心
- 价 格：¥49950
- 货 号：BioVector®-pSC189
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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BioVector® NTCC® pSC189细菌转座子递送载体BioVector® C9转座酶质粒
pSC189 BioVector®质粒是一种细菌转座子递送载体，用于随机插入诱变，特别是结合 mariner Himar1 转座子系统。
主要特性和信息 特性 详情载体类型转座子递送（自杀）载体大小6982 碱基对 (bp)宿主生物大肠杆菌（需要 $\lambda$pir 溶原宿主，如 SM10 $\lambda$pir 或 PIR1，才能复制）细菌抗性氨苄青霉素 (100 µg/mL) 和卡那霉素 (50 µg/mL)复制起点R6K \(\gamma \) 复制起点 ($ori$R6K) 和 RP4 转移起点 (\(oriT\))拷贝数低拷贝关键组件包含高活性的 Himar1 C9 转座酶基因、T3 和 T7 启动子以及 3xFLAG 表位标签研究中的应用 pSC189 质粒在接合实验中被用作“自杀载体”。因为它包含 R6K 复制起点，所以它只能在表达 $\lambda$pir 蛋白的大肠杆菌菌株中复制。当转移到缺乏这种蛋白的受体细菌物种中时，该质粒无法复制。然后，转座子（由 pSC189 主链携带）从非复制质粒跳入宿主基因组，从而实现稳定且可遗传的随机基因插入。 随机诱变：用于通过将转座子随机插入各种革兰氏阴性细菌（包括弧菌属和钩端螺旋体属物种）的基因组中来生成大型突变体库。基因发现：筛选所得的文库，以鉴定必需基因或参与特定过程（如毒力、应激抗性或核酸酶活性）的基因。表位标记：该载体旨在促进使用 3xFLAG 表位标签标记蛋白质，有助于功能研究和蛋白质表征。
The BioVector® pSC189 plasmid is a bacterial transposon delivery vector used for random insertional mutagenesis, particularly with the mariner Himar1 transposon system.
Key Features and Information Feature DetailVector TypeTransposon delivery (suicide) vectorSize6982 base pairs (bp)Host OrganismE. coli (requires a $\lambda$pir lysogen host like SM10 $\lambda$pir or PIR1 for replication)Bacterial ResistanceAmpicillin (100 µg/mL) and Kanamycin (50 µg/mL)Origin of ReplicationR6K \(\gamma \) origin of replication ($ori$R6K) and RP4 origin of transfer (\(oriT\))Copy NumberLow CopyKey ComponentsContains the hyperactive Himar1 C9 transposase gene, T3 and T7 promoters, and 3xFLAG epitope tagUsage in Research The pSC189 plasmid is used as a "suicide vector" in conjugation experiments. Because it contains the R6K origin of replication, it can only replicate in E. coli strains that express the $\lambda$pir protein. When transferred into a recipient bacterial species that lacks this protein, the plasmid cannot replicate. The mariner transposon (which is carried on the pSC189 backbone) then jumps from the non-replicating plasmid into the host's genome, allowing for random gene insertions that are stable and heritable. Random Mutagenesis: Used to generate large libraries of mutants by random insertion of the transposon into the genome of various Gram-negative bacteria, including Vibrio and Leptospira species.Gene Discovery: The resulting libraries are screened to identify essential genes or genes involved in specific processes like virulence, stress resistance, or nuclease activity.Epitope Tagging: The vector is designed to facilitate the tagging of proteins with a 3xFLAG epitope tag, aiding in functional studies and protein characterization.
Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net
pSC189 BioVector®质粒是一种细菌转座子递送载体，用于随机插入诱变，特别是结合 mariner Himar1 转座子系统。
主要特性和信息 特性 详情载体类型转座子递送（自杀）载体大小6982 碱基对 (bp)宿主生物大肠杆菌（需要 $\lambda$pir 溶原宿主，如 SM10 $\lambda$pir 或 PIR1，才能复制）细菌抗性氨苄青霉素 (100 µg/mL) 和卡那霉素 (50 µg/mL)复制起点R6K \(\gamma \) 复制起点 ($ori$R6K) 和 RP4 转移起点 (\(oriT\))拷贝数低拷贝关键组件包含高活性的 Himar1 C9 转座酶基因、T3 和 T7 启动子以及 3xFLAG 表位标签研究中的应用 pSC189 质粒在接合实验中被用作“自杀载体”。因为它包含 R6K 复制起点，所以它只能在表达 $\lambda$pir 蛋白的大肠杆菌菌株中复制。当转移到缺乏这种蛋白的受体细菌物种中时，该质粒无法复制。然后，转座子（由 pSC189 主链携带）从非复制质粒跳入宿主基因组，从而实现稳定且可遗传的随机基因插入。 随机诱变：用于通过将转座子随机插入各种革兰氏阴性细菌（包括弧菌属和钩端螺旋体属物种）的基因组中来生成大型突变体库。基因发现：筛选所得的文库，以鉴定必需基因或参与特定过程（如毒力、应激抗性或核酸酶活性）的基因。表位标记：该载体旨在促进使用 3xFLAG 表位标签标记蛋白质，有助于功能研究和蛋白质表征。
The BioVector® pSC189 plasmid is a bacterial transposon delivery vector used for random insertional mutagenesis, particularly with the mariner Himar1 transposon system.
Key Features and Information Feature DetailVector TypeTransposon delivery (suicide) vectorSize6982 base pairs (bp)Host OrganismE. coli (requires a $\lambda$pir lysogen host like SM10 $\lambda$pir or PIR1 for replication)Bacterial ResistanceAmpicillin (100 µg/mL) and Kanamycin (50 µg/mL)Origin of ReplicationR6K \(\gamma \) origin of replication ($ori$R6K) and RP4 origin of transfer (\(oriT\))Copy NumberLow CopyKey ComponentsContains the hyperactive Himar1 C9 transposase gene, T3 and T7 promoters, and 3xFLAG epitope tagUsage in Research The pSC189 plasmid is used as a "suicide vector" in conjugation experiments. Because it contains the R6K origin of replication, it can only replicate in E. coli strains that express the $\lambda$pir protein. When transferred into a recipient bacterial species that lacks this protein, the plasmid cannot replicate. The mariner transposon (which is carried on the pSC189 backbone) then jumps from the non-replicating plasmid into the host's genome, allowing for random gene insertions that are stable and heritable. Random Mutagenesis: Used to generate large libraries of mutants by random insertion of the transposon into the genome of various Gram-negative bacteria, including Vibrio and Leptospira species.Gene Discovery: The resulting libraries are screened to identify essential genes or genes involved in specific processes like virulence, stress resistance, or nuclease activity.Epitope Tagging: The vector is designed to facilitate the tagging of proteins with a 3xFLAG epitope tag, aiding in functional studies and protein characterization.
Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net
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