pgsA-745仓鼠卵巢细胞合成糖胺聚糖（GAGs）缺陷细胞株-BioVector NTCC典型培养物保藏中心

• Genetic origin: The pgsA-745 cell line was created by treating wild-type CHO-K1 cells with a mutagen (ethylmethanesulfonate) and then screening for mutants with defects in proteoglycan synthesis.

• Xylosyltransferase deficiency: The primary defect is a deficiency in xylosyltransferase (XylT), an enzyme essential for adding the first sugar residue in the synthesis of GAG chains, including heparan sulfate (HS) and chondroitin sulfate (CS). Due to this, the cells are unable to produce GAGs.

• Secondary mutation: More recent whole-genome sequencing has revealed that pgsA-745 cells also carry a secondary mutation: a deletion in the gene for laminin subunit $\alpha$2 (Lama2). This deletion affects the cell's ability to be invaded by certain bacteria, a trait initially attributed only to the GAG deficiency.

• Female origin: The cell line was derived from the ovary of a female Chinese hamster.

• Spontaneously immortalized: Like its parent line, pgsA-745 is an immortalized cell line.

The pgsA-745 cell line is widely used in biomedical research to study the functions of GAGs and proteoglycans. Some applications include:

• Cell adhesion and signaling: Studying the role of proteoglycans in cell-extracellular matrix interactions.

• Pathogen invasion studies: Investigating how the absence of GAGs affects cell invasion by various pathogens, including bacteria and viruses.

• Recombinant protein production: Used as a host cell line, particularly for producing proteins that do not require GAGs for proper function. The line is also studied for its resistance to infection.

• Glycosaminoglycan biology: Characterizing the specific roles of different GAGs in various cellular processes by comparing the mutant cells with wild-type CHO-K1 cells.

• Biosafety level: 1.

• Cryostorage: For long-term storage, frozen vials must be stored in the vapor phase of liquid nitrogen to preserve viability.

• Resuscitation: Upon thawing, the cryoprotective agent should be removed promptly via a quick spin and the cells resuspended in the appropriate complete growth medium.

• Medium and atmosphere: A complete medium, such as Ham's F12 with 10% fetal bovine serum, is used, along with a humidified 5% CO2 atmosphere.

BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

BioVector NTCC Inc.

TEL: 400-800-2947, 189-0126-8599

E-mail: biovector@163.com

http://www.biovector.net

• 遗传起源：pgsA-745 细胞系通过用诱变剂（乙基甲磺酸）处理野生型 CHO-K1 细胞，然后筛选蛋白聚糖合成缺陷的突变体而创建。

• 木糖基转移酶缺陷：主要缺陷是木糖基转移酶（XylT）的缺乏。该酶是合成 GAG 链（包括肝素硫酸和硫酸软骨素）中添加第一个糖残基所必需的。因此，该细胞无法产生 GAGs。

• 次要突变：最近的全基因组测序显示，pgsA-745 细胞还携带一个次要突变：层粘连蛋白亚基 $\alpha$2（Lama2）基因的缺失。这一缺失会影响细胞被某些细菌侵入的能力，这一特征最初只被归因于 GAG 缺陷。

• 雌性起源：该细胞系源自雌性中国仓鼠的卵巢。

• 自发永生化：与其亲本细胞系一样，pgsA-745 是一种永生化细胞系。

• 细胞黏附和信号传导：研究蛋白聚糖在细胞-细胞外基质相互作用中的作用。

• 病原体入侵研究：研究 GAGs 的缺失如何影响各种病原体（包括细菌和病毒）对细胞的入侵。

• 重组蛋白生产：用作宿主细胞系，特别是用于生产不需要 GAGs 发挥正常功能的蛋白。该细胞系也因其抗感染性而受到研究。

• 糖胺聚糖生物学：通过比较突变细胞和野生型 CHO-K1 细胞，表征不同 GAGs 在各种细胞过程中的具体作用。

• 生物安全级别：1 级。

• 冻存：为了长期保存，冷冻管必须储存在液氮气相中，以保持细胞活力。

• 复苏：解冻后，应通过快速离心立即去除冷冻保护剂，并将细胞重新悬浮在适当的完全生长培养基中。

• 培养基和培养环境：使用含 10% 胎牛血清的 Ham's F12 等完全培养基，以及 5% CO2 的湿润气氛。

BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

BioVector NTCC Inc.

TEL: 400-800-2947, 189-0126-8599

E-mail: biovector@163.com

http://www.biovector.net