BRL 3A大鼠自发永生化肝脏细胞spontaneously immortalized rat liver cell line -BioVector NTCC典型培养物保藏中心

NTCC® BRL 3A cell line

• 来源：雄性布法罗大鼠的正常肝脏。

• 细胞类型：上皮样，但也常被描述为成纤维细胞样。

• 生长特性：贴壁生长，在培养中具有高生长率。

• 独特之处：该细胞系产生的无血清条件培养基中含有一系列多肽，被称为“增殖刺激活性”（MSA）。这种活性可以部分满足其他细胞（如鸡胚成纤维细胞）对血清的需求。

• 培养条件：通常在添加胎牛血清（FBS）的Ham's F12培养基中培养。

• 生物安全等级：1级，对实验室人员和环境的风险较低。

• 肝脏学研究：作为研究肝细胞功能和肝再生机制的模型。

• 毒理学筛选：用于测试毒素、致癌物和纳米颗粒对肝功能的影响。

• 药物代谢：研究肝脏如何代谢各种药物和其他化合物。

• 细胞生物学：研究生长因子、细胞因子和基因修饰对肝细胞的影响。

• 病毒感染研究：研究与肝脏相关的病毒感染。

• 非人类模型：作为大鼠来源的细胞系，其研究结果可能无法完全直接应用于人类肝脏生理学。因此，通常需要通过其他实验模型来验证研究发现。

• 批次差异：尽管BRL 3A与亲本布法罗大鼠肝脏（BRL）细胞系相关，但研究表明，不同的BRL克隆（如3A和3A2）可能表现出不同的行为。

• 污染风险：与任何细胞培养一样，存在支原体和与其他细胞系交叉污染的风险。信誉良好的细胞系供应商会对这些问题进行筛选。

• Origin: Liver of a male Buffalo rat.

• Cell type: Epithelial-like, but has also been described as fibroblast-like.

• Growth properties: Adherent, with a high growth rate in culture.

• Unique feature: Produces a conditioned medium containing a family of polypeptides known as Multiplication Stimulating Activity (MSA). This activity helps partially satisfy the serum requirement for other cells, like chick embryo fibroblasts.

• Culture conditions: Typically cultured in Ham's F12 medium supplemented with Fetal Bovine Serum (FBS).

• Biosafety level: 1, indicating a low risk to laboratory personnel and the environment.

• Hepatology research: As a model for studying hepatocyte function and mechanisms of liver regeneration.

• Toxicology screening: Used to test the effects of toxins, carcinogens, and nanoparticles on liver function.

• Drug metabolism: Investigations into how the liver metabolizes various drugs and other compounds.

• Cellular biology: Studying the effects of growth factors, cytokines, and genetic modifications on liver cells.

• Viral infections: Research on liver-specific viral infections.

• Non-human model: As a rat-derived cell line, results may not always translate directly to human liver physiology. It is often necessary to corroborate findings with other experimental models.

• Variability: While BRL 3A is related to the parental Buffalo Rat Liver (BRL) line, studies have shown that different BRL clones, such as 3A and 3A2, can exhibit distinct behaviors.

• Contamination: As with any cell culture, there is a risk of mycoplasma and cross-contamination with other cell lines. Reputable cell line providers screen for these issues.

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