OKF6/TERT-2 NTCC™人口腔上皮角质形成永生化细胞株human oral epithelial keratinocytes cell line -BioVector NTCC典型培养物保藏中心

The NTCC™ OKF6/TERT-2 cell line is a human cell line of oral epithelial keratinocytes that has been immortalized through the introduction of the hTERT (human telomerase reverse transcriptase) gene. This makes it a valuable and reproducible model for studying the normal oral mucosa.

Key Characteristics

• Origin: The parent line, OKF6, was derived from the floor of the mouth of a 57-year-old male. The TERT-2 designation indicates a specific immortalized sub-line.

• Immortalization: Unlike primary cells, which have a limited lifespan, the OKF6/TERT-2 cell line expresses telomerase, which allows it to proliferate indefinitely without becoming cancerous. This provides a stable and consistent source of cells for long-term studies.

• Genetic Profile: A key feature is a homozygous deletion of the p16INK4a and p14ARF genes. These are well-known tumor suppressor genes, and their absence contributes to the cell line's extended lifespan.

• Physiological Relevance: The cells are able to differentiate in vitro into a multilayered epithelial structure that closely resembles the normal human oral mucosa, complete with a basal layer, stratum spinosum, and a non-keratinizing stratum corneum. This ability to form a tissue-like structure makes it a more physiologically relevant model than many other cell lines.

Research Applications

The OKF6/TERT-2 cell line is an important tool in various fields, particularly for studies that require a reliable model of the oral cavity. Its main applications include:

• Toxicology & Safety Testing: It is used to evaluate the irritant, inflammatory, or damaging effects of consumer products like toothpastes, mouthwashes, and e-liquid aerosols on the oral mucosa.

• Oral Biology & Disease Modeling: Researchers use it to study the molecular biology of diseases affecting the oral cavity, such as periodontal disease and oropharyngeal malignancy. It is also used for wound-healing assays to understand tissue repair mechanisms.

• Drug & Gene Delivery: Its ability to form a functional epithelial barrier makes it useful for testing the delivery of drugs or genes through the oral mucosa.

• 3D Organotypic Models: The cell line is a core component of advanced 3D organotypic models, where it is cultured with fibroblasts to create a full-thickness tissue analog that mimics the native oral mucosa. This allows for the study of complex cell-cell interactions.

NTCC™ OKF6/TERT-2 细胞系是一种人类口腔上皮角质形成细胞系，通过导入 hTERT（人类端粒酶逆转录酶）基因而实现了永生化。这使其成为研究正常口腔黏膜的宝贵且可重复的模型。

主要特点

• 来源: 其亲本细胞系 OKF6 源自一名57岁男性的口底组织。TERT-2 的命名表示它是一个特定的永生化亚系。

• 永生化: 与寿命有限的原代细胞不同，OKF6/TERT-2 细胞系表达端粒酶，使其能够无限期增殖而不会癌变。这为长期研究提供了稳定且一致的细胞来源。

• 遗传学特征: 一个关键特征是p16INK4a 和 p14ARF 基因的纯合性缺失。这些是著名的抑癌基因，它们的缺失有助于延长细胞系的寿命。

• 生理相关性: 这些细胞能够在体外分化形成多层上皮结构，与正常人类口腔黏膜非常相似，包含基底层、棘层和非角化性角质层。这种形成组织样结构的能力使其比许多其他细胞系更具生理相关性。

研究应用

OKF6/TERT-2 细胞系是各个领域的重要工具，尤其适用于需要可靠的口腔模型的研究。其主要应用包括：

• 毒理学与安全性测试: 它被用于评估牙膏、漱口水和电子烟气溶胶等消费品对口腔黏膜的刺激、炎症或损害作用。

• 口腔生物学与疾病建模: 研究人员使用它来研究影响口腔的疾病（如牙周病和口咽部恶性肿瘤）的分子生物学。它还用于伤口愈合试验，以了解组织修复机制。

• 药物与基因递送: 其形成功能性上皮屏障的能力使其适用于测试药物或基因通过口腔黏膜的递送效果。

• 3D 类器官模型: 该细胞系是先进3D 类器官模型的核心组成部分，它与成纤维细胞共同培养，以创建模拟原生口腔黏膜的全厚度组织模拟物。这使得研究复杂的细胞间相互作用成为可能。

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