NTCC™ GIPR-CHO-K1葡萄糖依赖性促胰岛素多肽受体过表达细胞株-BioVector NTCC典型培养物保藏中心

NTCC™ CHO-K1 GIPR overexpression cell line is a stable, genetically engineered cell line used in research to study the function of the GIP receptor (GIPR). These cells are created by introducing a gene that encodes for the GIPR into the parental CHO-K1 cell line, which normally doesn't express this receptor. This allows for controlled and high-level expression of GIPR.

What are the components of the cell line?

• CHO-K1 Cells: These are epithelial-like cells derived from the ovary of a Chinese hamster. They are a popular host for this kind of work because they are robust, easy to culture, and can be grown in large quantities.

• GIPR: This stands for glucose-dependent insulinotropic polypeptide receptor. It's a G protein-coupled receptor (GPCR) that plays a critical role in regulating glucose homeostasis. When the hormone GIP binds to its receptor, it stimulates the release of insulin from pancreatic beta-cells.

• Overexpression: The term "overexpression" means that the cells are engineered to produce a much larger amount of the GIPR protein than a normal cell would. This amplifies the cellular response to GIP, making it easier to study the receptor's function and its interaction with various ligands.

Why are these cell lines used?

These specialized cell lines are invaluable tools in drug discovery and basic research, particularly in the fields of metabolism and endocrinology. They are primarily used for:

• Drug Screening: Researchers use these cells to test the efficacy of new drug candidates for diseases like type 2 diabetes and obesity. By treating the cells with potential drugs, scientists can measure how effectively they activate or inhibit the GIPR. This is often done by detecting changes in intracellular signaling molecules, such as cAMP.

• Functional Assays: They provide a controlled environment to study the downstream signaling pathways of the GIPR. This helps in understanding how the receptor works and how its function can be modulated.

• Receptor Characterization: The cells are used to investigate the binding and activation properties of GIP and GIP-like molecules.

The "human/mouse/rat" designation simply refers to the species of the GIPR gene that was inserted into the CHO-K1 host cell. This allows researchers to study the GIPR protein from different species, which is crucial for preclinical studies and understanding species-specific differences in drug responses.

NTCC™ GIPR-CHO-K1 葡萄糖依赖性促胰岛素多肽受体过表达细胞株是一种稳定、经过基因工程改造的细胞系，用于研究 GIP 受体 (GIPR) 的功能。这些细胞是通过将编码 GIPR 的基因引入通常不表达该受体的亲本 CHO-K1 细胞系中而创建的。这使得研究人员能够可控地、高水平地表达 GIPR。

细胞系的组成部分是什么？

• CHO-K1 细胞: 这是源自中国仓鼠卵巢的上皮样细胞。它们是这类工作的常用宿主，因为它们强健、易于培养且可以大量生长。

• GIPR: 这代表葡萄糖依赖性促胰岛素多肽受体（glucose-dependent insulinotropic polypeptide receptor）。它是一种 G 蛋白偶联受体 (GPCR)，在调节葡萄糖稳态方面起着关键作用。当激素 GIP 与其受体结合时，会刺激胰腺 β 细胞释放胰岛素。

• 过表达: “过表达”一词意味着这些细胞经过改造，产生的 GIPR 蛋白质数量远高于正常细胞。这会放大细胞对 GIP 的反应，使其更容易研究受体的功能及其与各种配体的相互作用。

为什么使用这些细胞系？

这些特化的细胞系是药物发现和基础研究的宝贵工具，尤其是在代谢和内分泌学领域。它们主要用于：

• 药物筛选: 研究人员使用这些细胞来测试针对2型糖尿病和肥胖症等疾病的新药候选物的有效性。通过用潜在药物处理细胞，科学家可以测量它们激活或抑制 GIPR 的效果。这通常通过检测细胞内信号分子（如 cAMP）的变化来完成。

• 功能分析: 它们提供了一个受控环境来研究 GIPR 的下游信号通路。这有助于理解受体的工作方式及其功能如何被调节。

• 受体表征: 这些细胞用于研究 GIP 和类 GIP 分子的结合和激活特性。

BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

BioVector NTCC Inc.

TEL: 400-800-2947, 189-0126-8599

E-mail: biovector@163.com

http://www.biovector.net