甲烷氧化菌OB3b NTCC™ Methylosinus trichosporium OB3b BioVector NTCC典型培养物保藏中心

NTCC™甲烷氧化菌OB3b 指的是 Methylosinus trichosporium OB3b 菌株。这是一种重要的、经过深入研究的甲烷氧化细菌，被广泛用作该领域的模式生物。

主要特点

• 分类: 属于 II 型甲烷氧化菌，是一种好氧、革兰氏阴性细菌。

• 甲烷代谢: 这种细菌以甲烷作为唯一的碳源和能源。它通过两种关键酶——可溶性甲烷单加氧酶 (sMMO) 和颗粒性甲烷单加氧酶 (pMMO)——将甲烷氧化成甲醇。

• 铜离子调控 (Copper Switch): OB3b 菌株的一个显著特征是它会根据环境中铜离子的浓度来调节其甲烷单加氧酶的表达。

  • 低铜环境: 菌株会表达 sMMO。sMMO 是一种胞质酶，具有广泛的底物特异性，因此除了甲烷外，它还能降解多种有机污染物。

  • 高铜环境: 菌株则会表达 pMMO。pMMO 是一种膜结合酶，对甲烷具有更高的亲和力。

• 聚羟基丁酸酯 (PHB) 生产: 该菌株能够生产大量的 PHB，这是一种可降解的生物塑料。在某些研究中，OB3b 菌株的 PHB 含量可高达其干细胞重量的67%，是目前已知的甲烷氧化菌中最高的。

• 甲烷氧化菌素 (Methanobactin) 产生: 在铜离子缺乏时，OB3b 菌株会分泌一种被称为甲烷氧化菌素（Methanobactin）的铜螯合剂。这种小分子肽能够从环境中高效地捕获铜离子，并将其转运到细胞内，以激活 pMMO。

研究应用

Methylosinus trichosporium OB3b 菌株因其独特的代谢特性，在多个研究领域中具有重要价值：

• 生物技术: 利用其将甲烷转化为甲醇的能力，OB3b 菌株被用于开发将天然气或沼气转化为高价值化学品（如甲醇）或生物塑料（如 PHB）的生物技术方法。

• 环境修复: 由于 sMMO 具有降解多种卤代烃等污染物的能力，OB3b 菌株被研究用于生物修复被污染的土壤和地下水。

• 全球碳循环: 甲烷是一种强效温室气体。研究 OB3b 等甲烷氧化菌有助于我们理解它们在减少大气甲烷含量、减缓温室效应中的作用。

• 酶学研究: 它的双重甲烷单加氧酶系统是研究酶活性、金属离子调控和蛋白质结构与功能关系的理想模型。

BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

BioVector NTCC Inc.

TEL: 400-800-2947, 189-0126-8599

E-mail: biovector@163.com

http://www.biovector.net

NTCC™Methylosinus trichosporium OB3b is a well-studied strain of methanotrophic bacteria that serves as a model organism in the field. It's known for its ability to oxidize methane as its sole source of carbon and energy.

Key Characteristics

• Classification: It's a type II methanotroph, an aerobic, Gram-negative bacterium.

• Methane Metabolism: This strain uses methane as its only source of carbon and energy. It oxidizes methane to methanol using two key enzymes: soluble methane monooxygenase (sMMO) and particulate methane monooxygenase (pMMO).

• Copper Regulation (Copper Switch): A defining feature of the OB3b strain is its ability to regulate the expression of its methane monooxygenases based on the concentration of copper ions in the environment.

  • Low Copper: The strain expresses sMMO. This is a cytoplasmic enzyme with broad substrate specificity, allowing it to degrade various organic pollutants in addition to methane.

  • High Copper: The strain expresses pMMO. This is a membrane-bound enzyme with a higher affinity for methane.

• Polyhydroxybutyrate (PHB) Production: The strain can produce large amounts of PHB, a biodegradable bioplastic. In some studies, the PHB content of the OB3b strain has been found to be as high as 67% of its dry cell weight, one of the highest known among methanotrophs.

• Methanobactin Production: In the absence of copper, the OB3b strain secretes a small copper-chelating peptide called methanobactin. This molecule efficiently captures copper ions from the environment and transports them into the cell to activate pMMO.

Research Applications

Due to its unique metabolic properties, the Methylosinus trichosporium OB3b strain is valuable in several research areas:

• Biotechnology: Researchers use its ability to convert methane to methanol to develop biotechnological methods for converting natural gas or biogas into high-value chemicals like methanol or bioplastics like PHB.

• Environmental Remediation: Because sMMO can degrade various pollutants, including halogenated hydrocarbons, the OB3b strain is being studied for the bioremediation of contaminated soil and groundwater.

• Global Carbon Cycle: Methane is a potent greenhouse gas. Studying methanotrophs like OB3b helps us understand their role in reducing atmospheric methane levels and mitigating the greenhouse effect.


Licensed by Google

• Enzymology: Its dual methane monooxygenase system makes it an ideal model for studying enzyme activity, metal ion regulation, and the relationship between protein structure and function

BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

BioVector NTCC Inc.

TEL: 400-800-2947, 189-0126-8599

E-mail: biovector@163.com

http://www.biovector.net