CEM.NKR-CCR5-Luc用于艾滋病HIV/AIDS研究的人类 T 淋巴母细胞荧光表达株-BioVector NTCC典型培养物保藏中心

The NTCC™ CEM.NKR-CCR5-Luc cell line is a highly specialized tool in HIV/AIDS research, building upon the CEM.NKR-CCR5 cell line by adding a reporter gene.

Key Characteristics

• Parental Line: It is a derivative of the CEM.NKR-CCR5 cell line, which is in turn derived from the CEM.NKR line.

• Genetic Modification: This cell line has been genetically engineered to stably express two key components:

  1. CCR5: The human C-C chemokine receptor type 5, a critical coreceptor for R5-tropic HIV-1 entry.

  2. Luciferase Reporter Gene: This is the defining feature of the "Luc" variant. The luciferase gene is under the transcriptional control of the HIV-2 LTR (long terminal repeat).

• Mechanism of Action: When the CEM.NKR-CCR5-Luc cells are infected with HIV, the HIV Tat protein activates the HIV LTR promoter. This, in turn, drives the expression of the luciferase gene. By adding a luciferase substrate (like luciferin) to the culture, researchers can measure the amount of light produced, which directly correlates with the level of HIV replication. This provides a highly sensitive and quantifiable readout for infection.

• Susceptibility to HIV-1: Like its parent line, it expresses both CD4 and the coreceptors CCR5 and CXCR4. This makes it susceptible to a wide range of HIV-1 isolates, including both R5-tropic and X4-tropic strains.

Research Applications

The addition of the luciferase reporter gene makes the CEM.NKR-CCR5-Luc cell line extremely useful for high-throughput and quantitative assays, particularly for:

• HIV Neutralization Assays: This is its most common application. Researchers can use the cell line to test how well antibodies from patient serum or experimental drugs can neutralize HIV infection by measuring the reduction in luciferase activity.

• Antiretroviral Drug Screening: It is used to rapidly and quantitatively screen a large number of compounds for their ability to inhibit HIV replication. The luciferase readout provides a clear signal for a compound's antiviral effect.

• Studying HIV Entry Inhibitors: The cell line is an excellent model for studying drugs that specifically block HIV from entering cells by targeting the CCR5 or CXCR4 coreceptors.

• Vaccine Efficacy Testing: It is a valuable tool for assessing the effectiveness of HIV vaccine candidates by measuring their ability to elicit a neutralizing antibody response that prevents viral infection.

The use of this reporter system is a significant advancement over older methods, such as p24 antigen ELISA, because it is faster, more sensitive, and less labor-intensive, making it ideal for high-throughput screening.

NTCC™ CEM.NKR-CCR5-Luc 细胞系是艾滋病（HIV/AIDS）研究中一种高度专业化的工具，它在 CEM.NKR-CCR5 细胞系的基础上，通过添加报告基因而进一步优化。

主要特点

• 亲本细胞系: 它是 CEM.NKR-CCR5 细胞系的衍生品，而 CEM.NKR-CCR5 又源自 CEM.NKR 细胞系。

• 基因改造: 该细胞系经过基因工程改造，可稳定表达两个关键组分：

  1. CCR5: 人类 C-C 趋化因子受体5型，是 R5 嗜性 HIV-1 进入细胞的关键共受体。

  2. 荧光素酶报告基因 (Luc): 这是“Luc”变体的核心特征。该荧光素酶基因的表达受到 HIV-2 LTR（长末端重复序列）的转录控制。

• 作用机制: 当 CEM.NKR-CCR5-Luc 细胞被 HIV 感染后，HIV 的 Tat 蛋白会激活 HIV LTR 启动子。这反过来会驱动荧光素酶基因的表达。通过向培养物中添加荧光素酶底物（如荧光素），研究人员可以测量产生的发光量，这与 HIV 复制水平直接相关。这种机制为感染提供了高度敏感和可量化的读数。

• 对 HIV-1 的敏感性: 像其亲本细胞系一样，它同时表达 CD4 受体以及 CCR5 和 CXCR4 共受体。这使得它对广泛的 HIV-1 分离株（包括 R5 嗜性和 X4 嗜性毒株）都具有敏感性。

研究应用

荧光素酶报告基因的加入使得 CEM.NKR-CCR5-Luc 细胞系特别适合进行高通量和定量分析，主要用于：

• HIV 中和试验: 这是其最常见的应用。研究人员可以通过测量荧光素酶活性的降低程度，来测试来自患者血清的抗体或实验性药物中和 HIV 感染的效果。

• 抗逆转录病毒药物筛选: 它被用来快速、定量地筛选大量化合物，以评估它们抑制 HIV 复制的能力。荧光素酶的读数能清楚地显示化合物的抗病毒效果。

• 研究 HIV 进入抑制剂: 该细胞系是研究专门通过靶向 CCR5 或 CXCR4 共受体来阻断 HIV 进入细胞的药物的优秀模型。

• 疫苗效力测试: 它是评估 HIV 候选疫苗有效性的宝贵工具，通过测量它们引发中和抗体反应、从而阻止病毒感染这些细胞的能力。

这种报告系统相比于传统的 p24 抗原 ELISA 等旧方法是一个重大进步，因为它更快、更灵敏、工作量更小，非常适合高通量筛选。

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