CEM.NKR-CCR5用于艾滋病HIV/AIDS研究的人类 T 淋巴母细胞株-BioVector NTCC典型培养物保藏中心

The CEM.NKR-CCR5 cell line is a human T-lymphoblastoid cell line that is widely used in HIV/AIDS research. It's an engineered cell line, meaning it was modified from its parent line to express a specific protein.

Key Characteristics:

• Parent Cell Line: The original line is CEM.NKR, which is a clone of the well-known CEM cell line. A key feature of the parent CEM.NKR cells is their resistance to natural killer (NK) cell-mediated lysis.

• Genetic Modification: The CEM.NKR-CCR5 cells were created by transducing the CEM.NKR parent cells with a retroviral vector to make them express human CCR5.

• CCR5 Expression: This is the most crucial characteristic of this cell line. CCR5 (C-C chemokine receptor type 5) is a coreceptor that many strains of HIV-1 use to enter and infect T cells. The parental CEM.NKR cells do not express CCR5, making them resistant to R5-tropic HIV-1 strains. By introducing CCR5, the CEM.NKR-CCR5 cells become susceptible to infection by these strains.

• HIV-1 Susceptibility: The cell line expresses both the CD4 receptor and the CCR5 coreceptor (and also the CXCR4 coreceptor from its parent line), making it susceptible to infection by both R5-tropic and X4-tropic strains of HIV-1.

Research Applications:

The NTCC™ CEM.NKR-CCR5 cell line is an invaluable tool for virologists and immunologists, particularly in the study of HIV. Its primary applications include:

• HIV Neutralization Assays: This cell line is used to measure the ability of antibodies and other compounds (e.g., from patient serum) to neutralize or block HIV-1 infection. It's a key part of assays that assess how effectively a patient's immune system or a new drug can prevent the virus from entering cells.

• Antiretroviral Drug Screening: Researchers use CEM.NKR-CCR5 cells to screen for and evaluate the effectiveness of new anti-HIV drugs, especially those that target the CCR5 receptor to block viral entry.

• Studying HIV Pathogenesis: The cell line provides a consistent and reproducible model to study the early stages of HIV infection, including how the virus binds to receptors, enters the cell, and begins replication.

• Vaccine Research: It can be used to test the efficacy of vaccine candidates by measuring their ability to elicit a neutralizing antibody response that prevents HIV from infecting these cells.

NTCC™ CEM.NKR-CCR5 细胞系是一种广泛应用于艾滋病（HIV/AIDS）研究的人类 T 淋巴母细胞系。它是一种经过基因工程改造的细胞系，这意味着它是在其亲本细胞系的基础上，通过改造来表达某种特定蛋白质。

主要特点

• 亲本细胞系: 原始细胞系是 CEM.NKR，它是著名的 CEM 细胞系的一个克隆株。亲本 CEM.NKR 细胞的一个关键特征是它对自然杀伤（NK）细胞介导的裂解具有抵抗力。

• 基因改造: CEM.NKR-CCR5 细胞是通过逆转录病毒载体转导 CEM.NKR 亲本细胞而创建的，使其能够表达人类 CCR5。

• CCR5 表达: 这是该细胞系最重要的特性。CCR5 (C-C 趋化因子受体5型) 是许多 HIV-1 毒株用来进入并感染 T 细胞的共受体。亲本 CEM.NKR 细胞不表达 CCR5，因此对 R5 嗜性 HIV-1 毒株有抵抗力。通过引入 CCR5，CEM.NKR-CCR5 细胞变得容易被这些毒株感染。

• 对 HIV-1 的敏感性: 该细胞系同时表达 CD4 受体和 CCR5 共受体（以及来自其亲本细胞系的 CXCR4 共受体），因此它既能被 R5 嗜性 HIV-1 毒株感染，也能被 X4 嗜性 HIV-1 毒株感染。

研究应用

CEM.NKR-CCR5 细胞系是病毒学家和免疫学家宝贵的工具，尤其是在 HIV 研究中。其主要应用包括：

• HIV 中和试验: 该细胞系用于测量抗体和其他化合物（例如来自患者血清的物质）中和或阻断 HIV-1 感染的能力。它是评估患者免疫系统或新药物能否有效阻止病毒进入细胞的关键部分。

• 抗逆转录病毒药物筛选: 研究人员使用 CEM.NKR-CCR5 细胞来筛选和评估新型抗 HIV 药物的有效性，尤其是那些靶向 CCR5 受体以阻断病毒进入的药物。

• 研究 HIV 发病机制: 该细胞系提供了一个稳定且可重复的模型，用于研究 HIV 感染的早期阶段，包括病毒如何与受体结合、进入细胞并开始复制。

• 疫苗研究: 它可以用于测试候选疫苗的效力，通过测量它们引发中和抗体反应的能力，以防止 HIV 感染这些细胞。

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