| 出品公司: | -- |
|---|---|
| 载体名称: | pRI101 |
| 质粒类型: | 植物表达载体 |
| 高拷贝/低拷贝: | -- |
| 启动子: | -- |
| 克隆方法: | 多克隆位点,限制性内切酶 |
| 载体大小: | -- |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | -- |
| 筛选标记: | -- |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型: | -- |
| 病毒/非病毒: | -- |
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载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
pRI 101 DNA vectors are binary plant transformation vectors intended for foreign gene expression in plant cells. These agrobacterium-mediated plant transformation vectors carry the 35S promoter of caµliflower mosaic virus (CaMV) and the 5’ non-coding region (5’-UTR) of the alcohol dehydrogenase (ADH) gene. The 5’-UTR of ADH functions as a translation enhancer in plants. There are two types of pRI 101 DNA vectors, the pRI 101-AN DNA vector, which carries the 5’-UTR of Arabidopsis ADH (AtADH 5’-UTR), and the pRI 101 ON DNA vector, which carries the 5’-UTR of rice ADH (OsADH 5’-UTR). The pRI 101-AN DNA vector is for dicotyledonous plants such as tobacco or Arabidopsis while the pRI 101-ON DNA vector is for monocotyledonous plants such as rice.FeaturesAgrobacterium-mediated plant transformationEasy and stable integration of dicot and monocot target genes into plant chromosomesIncreased target gene expression via the 5'-UTR translation enhancer region Vector versatility for dicotyledonous and monoctyledonous plant cells载体序列
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