GM20390急性淋巴细胞白血病|易位染色体 B 淋巴细胞 LCL Cell Line细胞株-BioVector NTCC细胞库

Name细胞名称：NTCC® GM20390 ACUTE LYMPHOCYTIC LEUKEMIA | TRANSLOCATED CHROMOSOME B-Lymphocyte Cell Line
急性淋巴细胞白血病|易位染色体细胞株
Description描述：Clinically affected with pre-B acute lymphoblastic leukemia; donor subject suffered from general fatigue and anemia at age 3 years; bone-marrow examination at age 3 years showed 99% of blasts with French-American-British L1 morphology and pre-B-cell phenotype carrying a t(1;19)(q23;p13) translocation; early bone-marrow relapse occurred despite aggressive multidrug-combined chemotherapy; donor subject died from a fungal infection less than one year after diagnosis; cell line established from donor subject's peripheral blood at relapse; cells had basophilic cytoplasm with prominent vacuoles and oval nuclei with one or more prominent nuceoli; cells were negative for peroxidase, PAS, alpha-naphthyl butyrate esterase, and naphthol ASD chloroacetate esterase activities; cells expressed CD19, CD22, HLA-DR, and CD34 antigens but were negative for CD3, CD4, CD8, CD10, CD20, CD33, and surface immunoglobulins; cells harbored cIg and a rearranged Ig heavy-chain gene; cell line had neither E2A rearrangement nor PBX1 expression; cell line had no E2A/PBX1 chimeric transcripts; cloned breakpoints of cell line fell within introns of MEF2D and DAZAP1; the chimeric transcripts MEF2D-DAZAP1 and DAZAP1-MEF2D, whose sequences indicated in-frame fusions between MEF2D and DAZAP1, were expressed in the cell line and in bone-marrow cells from the donor subject.
Category分类：B-Lymphocyte
ID编号:NTCC®-GM20390
Size/Quantity数量: 1 Flask/2 Vials
Biosafety Level生物安全级别:1
Shipping Info运输方式: RT/Dry Ice
Storage储存方式: RT/Liquid Nitrogen
Race: Asian
Age年龄:4 YR
Gender性别:Female
Cell Type细胞类型: ACUTE LYMPHOCYTIC LEUKEMIA | TRANSLOCATED CHROMOSOME
Affected State:Yes
Product:LCL
Gene:
Mutations突变:
Ethnicity:
Family:
Relationship:proband
Karyotype:46,X,der(X;15)(15qter->15q11.2::Xp21->Xqter),t(1;19)(1pter->1q23::19p13->19pter;1qter->1q23::19p13->19qter),der(2)(2pter->2q35::22q11.2->22qter),t(3;5)(3pter->3q21::5q11.2->5qter;5pter->5q11.2::3q21->3qter),der(4)(21qter->21q11.2::4p13->4qter),der(9)(11pter->11p11.2::9p24->9qter),-9,del(11)(:p11.2->qter),-21,1~2mar[cp12].arr Xp22.33p11.22(168464-52911208)x1,Xp11.22q28(53809801-155233846)x3,9p24.1(4629337-5978120)x1,9p24.1p21.2(6873321-25872682)x1,9p21.2(26433904-29413903)x1,9q21.11q21.12(71361497-72904968)x1,9q22.33q31.1(100841489-104180857)x1,11p14.3p14.2(24446355-26566581)x1,11p12p11.2(43416303-44712996)x1,22q13.1q13.33(39576855-51234443)x3
Tissue Type组织来源:Blood
Complete Growth Medium完全培养基: BioVector® Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent Complete Medium
Subculturing传代方法:
Cryopreservation冻存方法:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions培养条件:Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
STR Profile鉴定数据:
References参考文献:

Supplier供应商:BioVector质粒载体菌株细胞蛋白抗体基因保藏中心
NTCC典型培养物保藏中心
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