NTCC® LβT2 LbetaT2 mouse pituitary gonadotrope immortalized cell line小鼠垂体促性腺素永生化细胞系-BioVector NTCC Inc.
- 价 格:¥99850
- 货 号:NTCC® LβT2 L beta T2; Lbeta T2; Lbetat2; LbT2
- 产 地:北京
- BioVector NTCC典型培养物保藏中心
- 联系人:Dr.Xu, Biovector NTCC Inc.
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地址:北京
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Application
Source.
The immortalized L(betta)T2 mouse pituitary gonadotrope cell line was generated by targeted tumorigenesis in transgenic mice carrying the rat LHb regulatory region linked to the SV40 T-antigen oncogene.1 L(betta)T2 cells express CGA, GnRH-R, and LHb. The cell line responds to pulsatile GnRH stimulation by upregulating Lhb and Gnrhr and secreting LH. The L(betta)T2 cell line has been widely used as an in vitro model for the study of gonadotropin gene regulation and GnRH signaling.2,3,4,5 The global mRNA expression profile and a genome-wide atlas of accessible chromatin in GnRH-stimulated L(betta)T2 cells has recently been described6 and the STR profile has also been reported.7
References
Note: Please include the appropriate references from 1-5 in your publications that utilize them.
1. Alarid ET, Windle JJ, Whyte DB, Mellon PL. Development 1996; 122(10):3319-3329.
2. Turgeon JL, Kimura Y, Waring DW, Mellon PL. Molecular Endocrinology 1996; 10(4):439-450.
3. Thomas P, Mellon PL, Turgeon J, Waring DW. Endocrinology 1996; 137(7):2929-2989.
4. Alarid ET, Holley S, Hayakawa M, Mellon PL. Mol Cell Endocrinol 1998; 140(1-2):25-30.
5. Pernasetti F, Vasilyev VV, Rosenberg SB, Bailey JS, Huang HJ, Miller WL, Mellon PL. Endocrinology 2001; 142(6):2284-2295.
6. Ruf-Zamojski F et al. Front Endocrinol (Lausanne) 2018; 9:34.
7. Ruf-Zamojski F et al. J Endocr Soc 2019; 3(5):902–920.
Features and Benefits
Storage and Stability
The cells should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Cell line name | LbetaT2 |
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Synonyms | L-beta-T2; L beta T2; Lbeta T2; L{beta}T2; Lbetat2; LbT2 |
Accession | CVCL_0398 |
Resource Identification Initiative | To cite this cell line use: LbetaT2 (RRID:CVCL_0398) |
Comments | Characteristics: Widely used as an in vitro model for the study of gonadotropin gene regulation and GnRH signaling (Millipore=SCC412). Characteristics: Expresses CGA, GnRH-R and the alpha and beta subunits of LH (PubMed=8770922). Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40). Derived from site: In situ; Pituitary gland; UBERON=UBERON_0000007. |
Species of origin | Mus musculus (Mouse) (NCBI Taxonomy: 10090) Breed/subspecies: C57BL/6 x BALB/c transgenic. |
Sex of cell | Female |
Category | Transformed cell line |
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