- BioVector NTCC典型培养物保藏中心
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邮件：Biovector@163.com
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BioVector® NTCC® pYL43
An NF-κB inducible SEAP expression vector driven by a promoter consisting of five NF-κB binding sequence (GGGGACTTTCC) and a minimal promoter containing a TATA box (TATATAA) (Pmin) (PNF-κB×5min-SEAP-pA). PNF-κB×5min was created by annealing OYL38 (5’- CTACGGGCTAGCTGGGGACTTTCCACTGGGGACTTTCCACTGGGGACTTTCCACTGGGGACTTTCCACTGGGGACTTTCCACTAGATCTAGACTCTAGAGGGTATATAATGGAAGCTCGAATTCCAGCTTGGCATTCCGGTACTGTTGGTAAAAAGCTTGGGGGG-3’, NheI and HindIII underlined) with OYL39 (5’-CCCCCCAAGCTTTTTACCAACAGTACCGGAATGCCAAGCTGGAATTCGAGCTTCCATTATATACCCTCTAGAGTCTAGATCTAGTGGAAAGTCCCCAGTGGAAAGTCCCCAGTGGAAAGTCCCCAGTGGAAAGTCCCCAGTGGAAAGTCCCCAGCTAGCCCGTAG -3’, HindIII and NheI underlined), digested with NheI/HindIII and ligated into pSEAP2-control (NheI/HindIII).
Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net
An NF-κB inducible SEAP expression vector driven by a promoter consisting of five NF-κB binding sequence (GGGGACTTTCC) and a minimal promoter containing a TATA box (TATATAA) (Pmin) (PNF-κB×5min-SEAP-pA). PNF-κB×5min was created by annealing OYL38 (5’- CTACGGGCTAGCTGGGGACTTTCCACTGGGGACTTTCCACTGGGGACTTTCCACTGGGGACTTTCCACTGGGGACTTTCCACTAGATCTAGACTCTAGAGGGTATATAATGGAAGCTCGAATTCCAGCTTGGCATTCCGGTACTGTTGGTAAAAAGCTTGGGGGG-3’, NheI and HindIII underlined) with OYL39 (5’-CCCCCCAAGCTTTTTACCAACAGTACCGGAATGCCAAGCTGGAATTCGAGCTTCCATTATATACCCTCTAGAGTCTAGATCTAGTGGAAAGTCCCCAGTGGAAAGTCCCCAGTGGAAAGTCCCCAGTGGAAAGTCCCCAGTGGAAAGTCCCCAGCTAGCCCGTAG -3’, HindIII and NheI underlined), digested with NheI/HindIII and ligated into pSEAP2-control (NheI/HindIII).
Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net
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