Magnaporthe oryzae B.C. Couch Guy11 strain稻瘟病菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心

Magnaporthe oryzae B.C. Couch Guy11 strain稻瘟病菌株

Magnaporthe oryzae Couch
BioVector®201236

An ampoule containing viable cells (may include spores and mycelia) suspended in cryoprotectant.

Product category
Fungi
Strain designation
Guy11
Type strain
No
Isolation source
Rice, Oryzae sativa
Geographical isolation
French Guiana
Product format
Frozen
Storage conditions
-80°C or colder

Characteristics
Mating type
MAT1-2
Comments
virulent on rice cultivar CO39
inheritance of virulence factors
Handling information
Medium
ATCC Medium 334: Oatmeal agar
Temperature
24-26°C
Atmosphere
Aerobic
Handling procedure
Frozen ampoules packed in dry ice should either be thawed immediately or stored in liquid nitrogen. If liquid nitrogen storage facilities are not available, frozen ampoules may be stored at or below -70°C for approximately one week. Do not under any circumstance store frozen ampoules at refrigerator freezer temperatures (generally -20°C). Storage of frozen material at this temperature will result in the death of the culture.
To thaw a frozen ampoule, place in a 25°C to 30°C water bath, until just thawed (approximately 5 minutes). Immerse the ampoule just sufficient to cover the frozen material. Do not agitate the ampoule.
Immediately after thawing, wipe down ampoule with 70% ethanol and aseptically transfer at least 50 µL (or 2-3 agar cubes) of the content onto a plate or broth with medium recommended.
Incubate the inoculum/strain at the temperature and conditions recommended. Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 5-10 days of incubation. However, the time necessary for significant growth will vary from strain to strain.

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