pMR103 plasmid vector噬菌粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pMR103 plasmid vector噬菌粒载体

pMR103
BioVector®87117


Organism
Escherichia coli (Migula) Castellani and Chalmers
Classification
Enterobacteriaceae, Escherichia
Clone type
Vector
Applications
Molecular biology

Specific applications
expression vector
vector permitting production of single-stranded DNA
Characteristics
Comments
Restriction digests of the clone give the following sizes (kb): PstI/SalI--5.2; PstI--5.2; SalI--uncut; EcoRV--3.2, 2.0; BstEII--3.6, 1.6; BstEII/PstI--3.0, 1.7, 0.5.
The SalI site was lost during cloning.
The lacI gene permits tight regulation of transcription of an insert and synthesis of potentially toxic proteins.
Expression vector (T7-based) with a kanR marker and a P15A replicon compatible with ColE1-derived plasmids. Particularly useful for co-transformation with ColE1-based ampR T7 expression vectors and the production of two proteins in the same cell.
If used in an Escherichia coli strain that expresses T7 polymerase under the control of the lacUV5 promotor (such as BL21(DE3)), addition of IPTG can result in high levels of recombinant protein production.
Mycoplasma contamination
Not detected
Vector information
Construct size (kb)
5.099999904632568
Intact vector size
4.100
Vector name
pMR102
Type of vector
phagemid
Construction
pMR101; pMR102, pET11d
Host range
Escherichia coli
Vector end
SalI; PstI
Vector information
Other unique sites: EcoNI, SalI, BstEII, PstI
Cloning sites
NcoI; BamHI
Markers
kanR
Operator
lac, <-, 3784-3800
Promoters
T7 (phi10), <-, 3804-3823
Replicon
M13, →, 11-467; p15A, →, 1585-1588
Repressor gene
lacI

Insert information
Insert size (kb)
1
Type of DNA
genomic
Genome
Escherichia coli
Gene name
lactose repressor
Gene product
lactose repressor [lacI]
Gene symbol
lacI
Contains complete coding sequence
Yes
Handling information
Host
Escherichia coli JM101 (ATCC 33876)
Medium
ATCC Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Temperature
37°C
Handling notes
Restriction digests of the clone give the following sizes (kb): PstI/SalI--5.2;
PstI--5.2; SalI--uncut; EcoRV--3.2, 2.0; BstEII--3.6, 1.6; BstEII/PstI--3.0,
1.7, 0.5. The SalI site was lost during cloning.
- ATCC staff

Expression vector (T7-based) with a kanR marker and a P15A replicon compatible
with ColE1-derived plasmids. Particularly useful for co-transformation with
ColE1-based ampR T7 expression vectors and the production of two proteins in the
same cell.
- Gene 144: 59-62, 1994

If used in an Escherichia coli strain that expresses T7 polymerase under the
control of the lacUV5 promotor (such as BL21(DE3)), addition of IPTG can result
in high levels of recombinant protein production.
- Gene 144: 59-62, 1994

The lacI gene permits tight regulation of transcription of an insert and
synthesis of potentially toxic proteins.
- Gene 144: 59-62, 1994

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