DHD/K12/TRb cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

DHD/K12/TRb cell line细胞株

Cell Line Name: DHD/K12/TRb

Keywords: Rat colonic carcinoma
Cell Line Description: This cell line has properties essential for study of metastasis of colon carcinomas; a reproducible pattern of metastatic behaviour and ability to be propagated both in vitro and in vivo to facilitate identification of biochemical correlates of metastasis.
Species: Rat
Tissue of Origin: colon
CellType: Epithelial
Growth Mode: Adherent
Karyotype: Not specified
Biosafety Information: Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

Hyperlinks to MSDS documents:
Frozen cell cultures Material Safety Data Sheet
Growing cell cultures Material Safety Data Sheet
Nucleic acids derived from cell cultures Material Safety Data Sheet
Subculture Routine: Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO2; 37°C.
Culture Medium: DMEM + F10 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) or 2% Rat Serum.

Supplier来源:BioVector NTCC Inc.
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