CARNAVAL cell line人弥漫性大B淋巴瘤细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

CARNAVAL cell line人弥漫性大B淋巴瘤细胞株

Cell line:
CARNAVAL
NTCC® no.:
NTCC®932724
Species validation:
human (Homo sapiens)
Cell type:
diffuse large B cell lymphoma
Origin:
established 2010 from the pleural effusion of a patient with diffuse large B cell lymphoma (DLBCL)

Biosafety level:
1
Permissions and restrictions:
A
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.

NTCC® Cell Culture Data:
Morphology:
single cells growing in suspension;
Image:


Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml; split saturated culture 1:2 to 1:3 every 2-3 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 40-50 hours
Harvest:
maximum density at ca. 1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO

NTCC® Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 -, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +;



Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human near diploid karyotype; 46(44-47)<2n>XX, +X, -Y, -4, +7, del(X)(p21)/idic r(X)(?p11?q21), der(8)t(8;11)(p23;?q23)t(11;17)(?q13;q11.2), t(8;14)(q24;q32), t(6;10)(q26;q23), der(11)t(11;16)(q13;q?11.2)dup(16)(q12q24), t(14;18)(q32;q21), del(16)(q21), der(17)t(4;17)(p11;q12)i(17)(q10); carries t(8;14) and t(14;18) effecting concurrent biallelic rearrangements of IGH with MYC and BCL2, respectively; resembles (unpublished) originator karyotype (by Siebert Lab, Kiel); heterozygosity of X-linked genes may indicate gender discrepancy regarding donor patient
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -

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