TWAR Strain CWL-029 Chlamydophila pneumoniae肺炎披衣菌VR-1310 BioVector NTCC质粒载体菌种细胞基因保藏中心

TWAR Strain CWL-029 Chlamydophila pneumoniae肺炎披衣菌VR-1310

rain CWL-029
Cat No.: NTCC-VR1310

Chlamydophila pneumoniae strain CWL-029 is propagated in HEp-2 cells (NTCC CCL-23). It was isolated from the throat swab of a pneumonia patient and deposited by the Centers for Disease Control and Prevention. Chlamydophila pneumoniae can be used in respiratory disease research.

Product category
Bacteria
Classification
Chlamydiaceae, Chlamydophila
Strain designation
CWL-029
Type strain
No
Isolation source
Throat swab of a pneumonia patient.
Applications
Infectious disease research
Respiratory disease research
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Specific applications
Respiratory research
Characteristics
Comments
Please see the NTCC Virology Guide for more detailed information on propagation
Mycoplasma contamination
Not detected
Technical information
NTCC Technical Services does not have technical information on patent deposits that are not produced or characterized by NTCC. Additional information can be found in the corresponding patent available from the patent holder or with the U.S. and/or international patent office.
Handling information
Host
HEp-2 (NTCC CCL-23)
Effects
CPE; cytoplasmic inclusions
Complete medium
Special Maintenance Medium (SMM): DMEM (NTCC 30-2002) + 10% FBS (NTCC 30-2020) + 10 mM HEPES (Gibco 15630) + 2 µg/mL Cycloheximide (SIGMA C4859)
Temperature
35°C
Atmosphere
95% Air, 5% CO2
Incubation
Incubate infected culture for 72 hours at 35°C in a humidified 5% CO2 atmosphere.
Recommendation for infection
For best results, infection should be performed on an 80-100% confluent, 24-48 hour old cellular monolayer.Sonicate seed material for 20 seconds at approximately 240W to disrupt cells. Prepare dilution of chlamydia in minimum amount of volume (e.g. 1 mL per 25 cm2). Remove cell growth medium and inoculate with disrupted material. For adsorption, centrifuge at 1500 x g at 25°C for 1 hour. End adsorption by adding agent growth medium.
Key abbreviations
DMEM, Dulbecco's Modified Eagle's Medium
FBS, Fetal bovine serum
g, Acceleration of gravity
HEPES, N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid)
HEp-2, Human laryngeal tumor cells
SMM, Special maintenance medium
Quality control specifications
Volume
1.0 mL

Level 2

Supplier来源:BioVector NTCC Inc.
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