Brevibacillus Expression System Ⅱ短芽孢杆菌表达系统 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥99865
- 货 号：Brevibacillus Expression System Ⅱ
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作QQ:1843439339 (微信同号)
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Brevibacillus Expression System Ⅱ短芽孢杆菌表达系统
DescriptionTheBrevibacillus (Bacillus brevis) Expression System is an efficient system for producing highyields of secreted protein. Using this system, it is possible to produce an unprecedentednumber of heterologous proteins. TheBrevibacillus Expression System offers the followingadvantages, and is particularly effective in the production of secretory proteins.・Extracellular secretion of large amounts of protein・Almost no detectable protease activity・Produces active proteins・Simple procedures for genetic manipulation and culturing・Safe host bacteriaExamples of protein production using this system are shown in Table 1. High expression levelswere obtained for a variety of proteins (enzymes, antigens, and cytokines), regardless of geneorigin (bacteria, archaea and eukaryotes). In particular, secretory proteins of eukaryotic origintypically contain S-S bonds for biological activity, and it is generally difficult to produce theseproteins using other prokaryotic expression systems. The characteristic secretory productionofBrevibacillus allows high-efficiency production, even for proteins with S-S bonds.It is possible to construct the expression vector using a shuttle vector inE. coli or by directlyintroducing the DNA ligation solution into the expression host. Target protein may be obtainedby culturing transformed host bacteria in test tubes or flasks (two types of media can beused; see Section VI. 9 for medium composition), and collecting protein from the culturesupernatant. Homogenization of bacteria is unnecessary; the target protein is obtained fromthe clear supernatant fraction following centrifugation (to pellet the bacterial cells) and maybe purified using standard techniques.ComponentsBrevibacillus Expression System II (Cat. #HB200)● Expression VectorspNY326 DNA 10 μg (Cat. #HB111)pNCMO2 DNA 10 μg (Cat. #HB112)● Control VectorpNY326-BLA DNA 1 μg (Cat. #HB114)● Competent CellsBrevibacillus Competent Cells (Cat. #HB116)・Brevibacillus Competent Cells 100 μl x 10・MT medium 1 ml x 10・Solution A 1 ml・Solution B 1 ml x 2
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
DescriptionTheBrevibacillus (Bacillus brevis) Expression System is an efficient system for producing highyields of secreted protein. Using this system, it is possible to produce an unprecedentednumber of heterologous proteins. TheBrevibacillus Expression System offers the followingadvantages, and is particularly effective in the production of secretory proteins.・Extracellular secretion of large amounts of protein・Almost no detectable protease activity・Produces active proteins・Simple procedures for genetic manipulation and culturing・Safe host bacteriaExamples of protein production using this system are shown in Table 1. High expression levelswere obtained for a variety of proteins (enzymes, antigens, and cytokines), regardless of geneorigin (bacteria, archaea and eukaryotes). In particular, secretory proteins of eukaryotic origintypically contain S-S bonds for biological activity, and it is generally difficult to produce theseproteins using other prokaryotic expression systems. The characteristic secretory productionofBrevibacillus allows high-efficiency production, even for proteins with S-S bonds.It is possible to construct the expression vector using a shuttle vector inE. coli or by directlyintroducing the DNA ligation solution into the expression host. Target protein may be obtainedby culturing transformed host bacteria in test tubes or flasks (two types of media can beused; see Section VI. 9 for medium composition), and collecting protein from the culturesupernatant. Homogenization of bacteria is unnecessary; the target protein is obtained fromthe clear supernatant fraction following centrifugation (to pellet the bacterial cells) and maybe purified using standard techniques.ComponentsBrevibacillus Expression System II (Cat. #HB200)● Expression VectorspNY326 DNA 10 μg (Cat. #HB111)pNCMO2 DNA 10 μg (Cat. #HB112)● Control VectorpNY326-BLA DNA 1 μg (Cat. #HB114)● Competent CellsBrevibacillus Competent Cells (Cat. #HB116)・Brevibacillus Competent Cells 100 μl x 10・MT medium 1 ml x 10・Solution A 1 ml・Solution B 1 ml x 2
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
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