A549-Dual™ KO-MDA5 Cells BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥98965
- 货 号：A549-Dual™ KO-MDA5 Cells
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作QQ:1843439339 (微信同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
- 已注册
A549-Dual™ KO-MDA5 Cells
MDA-5 knockout NF-kB-SEAP & IRF-Lucia Reporter Cell LineA549-Dual™ KO-MDA5 cells were generated from A549-Dual™ cells through the stable knockout of the MDA5 gene. They are adherent epithelial cells derived from the human A549 lung carcinoma cell line by stable integration of two inducible reporter constructs. The A549 cell line, a cellular model for asthma and respiratory infections, expresses many pattern recognition receptors (PRRs), including RIG-I [1, 2], and the Toll-like receptors (TLRs) TLR2 [3], TLR3 and TLR5 but not TLR4 [3].A549-Dual™ KO-MDA5 and A549-Dual™ cells express a secreted embryonic alkaline phosphatase (SEAP) reporter gene under the control of the IFN-β minimal promoter fused to five NF-κB binding sites. They also express the secreted Lucia luciferase reporter gene under the control of an ISG54 minimal promoter in conjunction with five IFN-stimulated response elements. As a result, they allow to simultaneously study the NF-kB pathway, by assessing the activity of SEAP, and the interferon regulatory factor (IRF) pathway, by monitoring the activity of Lucia luciferase. Both reporter proteins are readily measurable in the cell culture supernatant when using QUANTI- Blue™, a SEAP detection reagent, and QUANTI_x001e_Luc™, a Lucia luciferease detection reagent.A549-Dual™ KO-MDA5 cells are resistant to blasticidin and Zeocin™. References:1. Kolokoltsova OA. et al., 2014. RIG-I enhanced interferon independent apoptosis upon Junin virus infection. PLoS One. 9:e99610.2. Hagmann CA. et al., 2013. RIG-I detects triphosphorylated RNA of Listeria monocytogenes during infection in non-immune cells. PLoS One. 8:e62872.3. Slevogt H. et al., 2007. Moraxella catarrhalis is internalized in respiratory epithelial cells by a trigger-like mechanism and initiates a TLR2- and partly NOD1-dependent inflammatory immune response. Cell Microbiol. 9(3):694-707.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
MDA-5 knockout NF-kB-SEAP & IRF-Lucia Reporter Cell LineA549-Dual™ KO-MDA5 cells were generated from A549-Dual™ cells through the stable knockout of the MDA5 gene. They are adherent epithelial cells derived from the human A549 lung carcinoma cell line by stable integration of two inducible reporter constructs. The A549 cell line, a cellular model for asthma and respiratory infections, expresses many pattern recognition receptors (PRRs), including RIG-I [1, 2], and the Toll-like receptors (TLRs) TLR2 [3], TLR3 and TLR5 but not TLR4 [3].A549-Dual™ KO-MDA5 and A549-Dual™ cells express a secreted embryonic alkaline phosphatase (SEAP) reporter gene under the control of the IFN-β minimal promoter fused to five NF-κB binding sites. They also express the secreted Lucia luciferase reporter gene under the control of an ISG54 minimal promoter in conjunction with five IFN-stimulated response elements. As a result, they allow to simultaneously study the NF-kB pathway, by assessing the activity of SEAP, and the interferon regulatory factor (IRF) pathway, by monitoring the activity of Lucia luciferase. Both reporter proteins are readily measurable in the cell culture supernatant when using QUANTI- Blue™, a SEAP detection reagent, and QUANTI_x001e_Luc™, a Lucia luciferease detection reagent.A549-Dual™ KO-MDA5 cells are resistant to blasticidin and Zeocin™. References:1. Kolokoltsova OA. et al., 2014. RIG-I enhanced interferon independent apoptosis upon Junin virus infection. PLoS One. 9:e99610.2. Hagmann CA. et al., 2013. RIG-I detects triphosphorylated RNA of Listeria monocytogenes during infection in non-immune cells. PLoS One. 8:e62872.3. Slevogt H. et al., 2007. Moraxella catarrhalis is internalized in respiratory epithelial cells by a trigger-like mechanism and initiates a TLR2- and partly NOD1-dependent inflammatory immune response. Cell Microbiol. 9(3):694-707.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
- 公告/新闻
免费订购电话: 400-800-2947