BIONi010-A Human iPS Cell Line人诱导多能干细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

BIONi010-A Human iPS Cell Line人诱导多能干细胞株

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.

Derivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
Lin28
MYC
POU5F1
shP53
SOX2
Is the reprogramming vector detectable: Unknown
Other:From the same donor: BIONi010-B, BIONi010-C

Characterization
Analysis of Undifferentiated Cells
Marker expression: SOX2(+)SSEA-4(+)NANOG(+)POU5F1 (OCT-4)(+)SSEA-3(+)DNMT3B(+)TRA 1-60(+)
Differentiation potency
Ectoderm: Neuron
In vitro spontaneous differentiation
In vitro directed differentiation
Marker Expressed:Nestin(+)Tuj1 (+)vGLUT1(+)VIMENTIN(+)
Endoderm: In vitro spontaneous differentiation
Marker Expressed:AFP(+)
Mesoderm: In vitro spontaneous differentiation
Marker ExpressedSMA (+)

Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation

Genotyping
Karyotyping
Passage number: 20
Cell line karyotype: Normal
Karyotyping method: G-Banding
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.

Subculture Routine
Medium: mTeSR®
Passage method: EDTA
Matrix: Matrigel® / Geltrex®
CO2 concentration: 5%
O2 concentration: 5%
Temperature: 37°C

Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
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