GZUF36 Aspergillus niger strain黑曲霉菌株CCTCC No.M2012538胞内脂肪酶产生菌 BioVector NTCC质粒载体菌种细胞基因保藏中心

GZUF36 Aspergillus niger strain黑曲霉菌株CCTCC No.M2012538胞内脂肪酶产生菌

一种胞内脂肪酶产生菌及其应用及筛选方法及使用方法，所述的菌株保藏编号为CCTCC?No.M2012538。本发明从富油土壤中分离得到一种胞内脂肪酶产生菌

Strain Designations A. niger M2012538
Application Production of sn-2 selective lipase
Biosafety Level 1
Comments An aspergillus niger strain No. M2012538 with high yield of sn-2 selective lipase from soil in the earlier stage. Experiments show that the ratio of 1,3-DG/1,2-DG is higher than 6, the yield of 1,3-DG is higher than 16.34 percent, and the activity of the produced enzyme is 8u/mL when the enzyme fermentation liquor is used for hydrolyzing triglyceride. Different inducers, carbon sources, nitrogen sources and different culture methods have great influence on the expression of lipase-producing genes, so that the optimization of a culture medium and the selection of the culture method are extremely important. Researcher screens out a proper culture medium and a proper culture method, and increases the enzyme activity from the initial 8u/ml to 20u/ml, thereby laying the foundation for oil modification, wastewater treatment and infant food development.
Medium Czapek's medium
Medium 325: Malt extract agar (Blakeslee's formula)
Medium 28: Emmons' modification of Sabouraud's agar
Medium 336: Potato dextrose agar (PDA)
Growth Conditions Temperature: 24°C to 26°C
Atmosphere: Typical aerobic

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