ArcticE strain菌株/competent cells感受态细胞 BioVector NTCC质粒载体菌种细胞基因保藏中心

ArcticE strain菌株/competent cells感受态细胞

Derived from E. coli B, the ArcticExpress expression strains naturally lack
the Lon protease, which can degrade recombinant proteins. In addition,
these strains are engineered to be deficient for a second protease, the OmpT
protein. These strains also feature the Hte phenotype* present in Agilent’s
highest efficiency competent cell strain, XL10-Gold.4
The presence of the
Hte phenotype increases the transformation efficiency of the cells. In
addition, the gene that encodes endonuclease I (endA), an enzyme that
rapidly degrades plasmid DNA isolated by most miniprep procedures, has
been inactivated in these cells. These two features enable direct cloning of
many protein expression constructs. The Oleispira antarctica chaperonins
Cpn10 and Cpn60 are constitutively expressed in these strains from a
ColE1-compatible, pACYC-based plasmid that contains a gentamycinresistance gene

Genotype
ArcticExpress (DE3) strain E. coli B F–
ompT hsdS(rB
–
mB
–
) dcm+ Tetr gal λ(DE3) endA Hte [cpn10
cpn60 Gentr
]
ArcticExpress strain E. coli B F–
ompT hsdS(rB
–
mB
–
) dcm+ Tetr gal endA Hte [cpn10 cpn60 Gentr
]

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