S9.6杂交瘤细胞HB-8730 BioVector NTCC质粒载体菌种细胞基因保藏中心

S9.6杂交瘤细胞HB-8730

The mAb product of this cell line may be used for microarray detection without amplification of RNA; detection of R-loops.

Immunogen: DNA-RNA hybrids synthesized from φΧ174 single stranded-DNA.

Primary reactivity: DNA-RNA hybrids

Product category
Animal cells
Product type
Hybridoma
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell type
hybridoma: b lymphocyte
Morphology
lymphoblast
Applications
Immunology

Specific applications
This hybridoma produces a monoclonal antibody, mAb S9.6, which detects DNA/RNA hybrids.

Growth properties
Suspension
Derivation
Originally isolated by Carrico and colleagues in 1985. This hybridoma has been recloned and verified as making the intended monoclonal antibody.
Strain
BALB/c
Genes expressed
mAb, S9.6
Isotype
IgG2a, kappa light chain
Comments
Mouse (BALB/c) B-cell fused with Mouse (BALB/c) Sp2/0–Ag14 myeloma to produce monoclonal antibodies against RNA-DNA hybrids. Animals were immunized with RNA-DNA hybrids formed by using single stranded PhiX174 DNA as template for E. coli DNA dependent RNA polymerase.
The mAb product of this cell line may be used for microarray detection without amplification of RNA; detection of R-loops.

Immunogen: DNA-RNA hybrids synthesized from ?Χ174 single stranded-DNA.

Primary reactivity: DNA-RNA hybrids

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