pHS17 E. coli-F. nucleatum shuttle vector具核梭杆菌质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pHS17 E. coli-F. nucleatum shuttle vector具核梭杆菌质粒载体

Construction of a shuttle plasmid, pHS17, by using pFN1 has enabled the transformation of F. nucleatum (12). A current limitation of the pHS17 shuttle plasmid is that it has been successfully established only in two strains of F. nucleatum. Because alternative vector systems will be important in molecular analysis of fusobacteria, we isolated additional native plasmids from F. nucleatum. One plasmid, pKH9, was characterized and found to possess features not previously identified in fusobacterial plasmids. We describe here the minimal replicon that was used to construct a pKH9-based shuttle plasmid and studies that strongly indicate a fusobacterial plasmid addiction system.

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