pBSKII-tocas9 plasmid真菌基因编辑质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pBSKII-tocas9 plasmid真菌基因编辑质粒载体

a plasmid pBSKII-toCas9-hph containing the cas9 cassette under the control of A. nidulans trpC promoter and terminator was constructed and transformed into Nodulisporium sp. (No. 65-12-7-1) according to the established protoplast transformation procedure. Seven hygromycin-resistance fungus clones were randomly selected. Since the plasmid pBSKII-toCas9-hph we used for Cas9 expression does not contain a fungus origin of replication, integration of the plasmid into the genome is required for the growth of transformants in the presence of hygromycin. As expected, the integration of hygromycin resistance gene into the genomes of all seven clones was confirmed

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