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BRIN-BD11 cell line细胞株
Cell Line Name: BRIN-BD11Keywords: A rat pancreatic Beta cell: RINm5F hybrid cell line. Insulin-secreting cell lineCell Line Description: BRIN-BD11 is a hybrid cell line formed by the electrofusion of a primary culture of NEDH rat pancreatic islets with RINm5F (a cell line derived from a NEDH rat insuliinoma). BRIN-BD11 has been shown to be tumourigenic when transplanted into a SCID mouse host. BRIN-BD11 has been mycoplasma eradicated and the stocks available for supply have undergone a further 10 passages without detection of mycoplasma. The cell line has applications in the study of pancreatic beta cell function.Species: RatTissue of Origin: Pancreatic isletsCellType: EpitheloidKaryotype: Modal chromosome number 58 over 50 passagesSubculture Routine: Split sub-confluent cultures (70-80%) 1:8 to 1:10 i.e. seeding at 2-4 x 104 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C. Population doubling approx 20hrs. At confluence 105 cells/cm² can be expected.Culture Medium: RPMI-1640 + 2mM Glutamine + 10% FCSReferences: McClenaghan NH, Barnett CR, Ah-Sing E, Abdel-Wahab YH, O'Harte FP, Yoon TW, Swanston-Flatt SK, Flatt PR. 1996 Characterization of a novel glucose-responsive insulin-secreting cell line, BRIN-BD11, produced by electrofusion Diabetes. 45(8):1132-40. PMID: 8690162Additional Bibliography: McClenaghan NH, Flatt PR 1999 Engineering cultured insulin-secreting pancreatic B-cell lines. J Mol Med. 77(1):235-43 PMID: 9930971.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
Cell Line Name: BRIN-BD11Keywords: A rat pancreatic Beta cell: RINm5F hybrid cell line. Insulin-secreting cell lineCell Line Description: BRIN-BD11 is a hybrid cell line formed by the electrofusion of a primary culture of NEDH rat pancreatic islets with RINm5F (a cell line derived from a NEDH rat insuliinoma). BRIN-BD11 has been shown to be tumourigenic when transplanted into a SCID mouse host. BRIN-BD11 has been mycoplasma eradicated and the stocks available for supply have undergone a further 10 passages without detection of mycoplasma. The cell line has applications in the study of pancreatic beta cell function.Species: RatTissue of Origin: Pancreatic isletsCellType: EpitheloidKaryotype: Modal chromosome number 58 over 50 passagesSubculture Routine: Split sub-confluent cultures (70-80%) 1:8 to 1:10 i.e. seeding at 2-4 x 104 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C. Population doubling approx 20hrs. At confluence 105 cells/cm² can be expected.Culture Medium: RPMI-1640 + 2mM Glutamine + 10% FCSReferences: McClenaghan NH, Barnett CR, Ah-Sing E, Abdel-Wahab YH, O'Harte FP, Yoon TW, Swanston-Flatt SK, Flatt PR. 1996 Characterization of a novel glucose-responsive insulin-secreting cell line, BRIN-BD11, produced by electrofusion Diabetes. 45(8):1132-40. PMID: 8690162Additional Bibliography: McClenaghan NH, Flatt PR 1999 Engineering cultured insulin-secreting pancreatic B-cell lines. J Mol Med. 77(1):235-43 PMID: 9930971.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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