HUDEP-2 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

HUDEP-2 cell line细胞株

HUDEP-2 update : 2021/07/07
Comment Human erythroid progenitor cell line derived from umbilical cord blood cells. Expressing adult type hemoglobin.

Year of deposit 2013
Cloning (depositor) No
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Tissue umbilical cord blood
Classification transformed
Recombinant recombinant
Exogene KUSABIRA-ORANGE, HPV16 E6/E7
Vector CSIV-TRE-HPV-E6/E7-UbC-KT lentiviral vector
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_VI06
deposit info
lot info
Medium Medium List
Culture type Suspension cells Suspension cells
Medium and additives StemSpan SFEM (Stemcell technologies), EPO(3U/ml), SCF(50ng/ml), DEX(10-6M), DOX(1ug/ml) StemSpan SFEM +3U/ml EPO + 50ng/ml SCF + 10^(-6)M Dexamethasone + 1ug/ml Doxycycline
Antibiotics  Free
Passage method  dilution
Culture information Passage ratio  1 : 5-10 split
SC frequency Subculture : 2 times/week Subculture : 2 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freeze medium Medium + 10% DMSO CELLBANKER 1
Freezing method  Slow freezing
Mycoplasma  (-)
Animal PCR  OK
Virus (HIV)  Undetected
Virus (HTLV-1)  (-)
STR(human)  OK

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