phAE159，phAE87 shuttle phasmid噬菌粒 BioVector NTCC质粒载体菌种细胞基因保藏中心

phAE159，phAE87 shuttle phasmid噬菌粒

Two shuttle phasmids, phAE87 and phAE159, were constructed using the PacI excisable cosmid for efficient transposon mutagenesis. The shuttle phasmid phAE87 was used to make our first library of thousands of transposon mutants in M. tuberculosis with Tn5367 (an IS1096-derived transposon [106]). The shuttle phasmid was useful for generating transposon libraries in M. avium (121), Mycobacterium ulcerans (122), Mycobacterium paratuberculosis (123), and Mycobacterium abscessus

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