Ruminococcus lactaris 酸奶瘤胃球菌ATCC 29176

Ruminococcus lactaris Moore et al.

Cat# BioVector29176

Type strain

Product category

Bacteria

Product type

Anaerobe

Strain designation

VPI X6-29

Type strain

Yes

Isolation source

Feces

Product format

Freeze-dried

Medium 260: Trypticase soy agar/broth with defibrinated sheep blood

Temperature

37°C

Atmosphere

Anaerobic

Handling procedure

Open vial according to enclosed instructions.

Under anaerobic conditions aseptically rehydrate the entire pellet with approximately 0.5 mL of #1490 broth. Aseptically transfer the entire contents to a 5-6 mL tube of #1490 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary broth tubes. Best practice dictates the use of pre-reduced media.

Use several drops of the primary broth tube to inoculate a #260 plate and/or #260 agar slant.

Incubate in an anaerobic atmosphere at 37°C for 48-72 hours. Incubate one agar plate aerobically at 37°C to check for contamination.

ANAEROBIC CONDITIONS:

Anaerobic conditions for transfer may be obtained by the use of an anaerobic gas chamber or placement of test tubes under a gassing cannula system connected to anaerobic gas.

Anaerobic conditions for incubation may be obtained by any of the following:

Loose screw caps on test tubes in an anaerobic chamber

Loose screw caps on test tubes in an activated anaerobic gas pack jar

Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained

Handling notes

Anaerobe Systems Brucella Blood Agar plates (AS-111 or AS-141) are recommended for analyzing colony morphology and purity.

Deposited as

Ruminococcus lactaris Moore et al.

Depositors

LV Holdeman

Type of isolate

Human

Cross references

GenBank L76602 Ruminococcus lactaris small subunit ribosomal RNA (16S rDNA) gene.

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Ruminococcus lactaris

SubSpecies:  

Type Strain: BioVector29176

Risk Group: 1

REMARKS:  AUTHORS: Moore et al. 1976 RECORD No.: 784837

Supplier: BioVector NTCC Inc. http://www.biovector.net