5' RACE System for Rapid Amplification of cDNA Ends, version 2.0, 10 reactions BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥19865
- 货 号：5' RACE System for Rapid Amplification of cDNA Ends, version 2.0
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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5' RACE System for Rapid Amplification of cDNA Ends, version 2.0, 10 reactions
DescriptionThe 5´ RACE System for Rapid Amplification of cDNA Ends, Version 2.0, is suitable for rapid amplification of cDNA ends (RACE) between a defined point in the mRNA and the 5´ end. The 5' RACE System provides a set of prequalified reagents intended for synthesis of first-strand cDNA, purification of first-strand products, homopolymeric tailing, and preparation of target cDNA for subsequent amplification by PCR. Control RNA, DNA, and primers are provided for monitoring system performance. The system is useful for the amplification of rare messages for which little sequence information may be available, and for capturing the 5´ end information of mRNA. The 5´ RACE system:• Uses the advanced capabilities of SuperScript® II RNase H- RT for greater first-strand cDNA yields and increased cDNA lengths• Prepares specific cDNA from up to 1 µg of total or poly(A)+ RNA in one reaction• Offers improved first-strand cDNA purification procedure that yields cDNA suitable for tailing• Uses recombinant TdT with optimized reaction conditions in a PCR-compatible buffer to provide an efficient primer annealing site for PCRFor Use With (Application): cDNA Libraries & Library ConstructionProduct Type: cDNA libraryQuantity: 10 reactionsStorage Requirements: Store the reagents at -20°C and the DNA purification components at 4°C. All reagents guaranteed stable for 6 months when properly stored.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
DescriptionThe 5´ RACE System for Rapid Amplification of cDNA Ends, Version 2.0, is suitable for rapid amplification of cDNA ends (RACE) between a defined point in the mRNA and the 5´ end. The 5' RACE System provides a set of prequalified reagents intended for synthesis of first-strand cDNA, purification of first-strand products, homopolymeric tailing, and preparation of target cDNA for subsequent amplification by PCR. Control RNA, DNA, and primers are provided for monitoring system performance. The system is useful for the amplification of rare messages for which little sequence information may be available, and for capturing the 5´ end information of mRNA. The 5´ RACE system:• Uses the advanced capabilities of SuperScript® II RNase H- RT for greater first-strand cDNA yields and increased cDNA lengths• Prepares specific cDNA from up to 1 µg of total or poly(A)+ RNA in one reaction• Offers improved first-strand cDNA purification procedure that yields cDNA suitable for tailing• Uses recombinant TdT with optimized reaction conditions in a PCR-compatible buffer to provide an efficient primer annealing site for PCRFor Use With (Application): cDNA Libraries & Library ConstructionProduct Type: cDNA libraryQuantity: 10 reactionsStorage Requirements: Store the reagents at -20°C and the DNA purification components at 4°C. All reagents guaranteed stable for 6 months when properly stored.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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