CMV‐HTLV‐I R RNA Pol II promoter BioVector NTCC质粒载体菌种细胞基因保藏中心

CMV‐HTLV‐I R RNA Pol II promoter

Both CMV‐HTLV‐I R RNA Pol II promoter (protein transgene) and murine U6 RNA Pol III promoter (RNA transgene) vector designs were studied. Plasmid production yield was assessed through inducible fed‐batch fermentation. RNA Pol II‐directed enhanced green fluorescent protein and RNA Pol III‐directed RNA expression were quantified by fluorometry and quantitative real‐time polymerase chain reaction, respectively, after transfection of human HEK293 cells.

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