dh5α attλ::p5/66/6-rna-in-sacb, catR E.coli strain大肠杆菌菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心

dh5α attλ::p5/66/6-rna-in-sacb, catR E.coli strain大肠杆菌菌株

grown in NTC4862 (DH5α attλ:P5/6 6/6-RNA-IN-SacB,. CmR) or XL1-Blue ... CAT RNA-IN-SacB (P5/66/6) integration plasmid. The NTC8685-eGFP vector may be cloned on its host (NTC4862 (DH5α
attλ::P5/66/6-RNA-IN-SacB,catR)) and confirmed with ten colonies were selected randomly
from selection medium and confirmed using colony-PCR single 700 bp band. Plasmid from
Clone-10 was extracted and confirmed using PCR result a single 700 bp band. Plasmid was
also confirmed by restriction analysis using BglII-SalI double digest that result in two distinct
bands: 3000 bp and 700 bp band. Then, the best NTC8685-eGFP chitosan-formulation
formulation was obtained 1: 1.0, so the NTC8685-eGFP vector could be a vector candidate
for vector DNA vaccine with the capsuleation of chitosan nanoparticles.


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