W303 yeast strain酵母菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心

W303 yeast strain酵母菌株

W303 Genotype: MATa/MATα {leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15} [phi+] allele locus mutation (1) ade2-1 YOR128C nonsense, glu64STOP trp1-1 YDR007W nonsense, glu83STOP can1-100 YEL063C frameshift, lys47 leu2-3,112 YCL018W frameshift, gly83 his3-11,15 YOR202W 2x frameshifts, ala70 and glu106 Notes: The W303 genome is to 85.4% derived from S288c, part of the other regions are similar to non-S288c regions of Sigma1278b. In total, some 800 CDS differ between W303 and S288c, but in most cases only one or two residues differ [2]. These include a bud4 mutation that causes haploids to bud with a mixture of axial and bipolar budding patterns. In addition, the original W303 strain contains the rad5-535 allele. As S288c, W303 has an allelic variant of MIP1 which increases petite frequency. The W303 genome was sequenced at the Sanger Institute and by Ralser M. et al. (2012) Open Biol 2: 120093. 1 (DDBJ/EMBL/GenBank ALAV00000000). References: W303 constructed by Rodney Rothstein (see detailed notes from RR and Stephan Bartsch). bud4 info: Original mutant description Voth et al. (2005) Eukaryotic Cell, 4:1018-28. Mutation: deletion of one of four Gs at positions 2456-2459 of BUD4 ORF. Seq data from: Ralser et al above rad5-535 info: see detailed notes Sources: ATCC:200060

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